ArticleMolecular and cellular biology1996
Three functional classes of transcriptional activation domain.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 266 citations in OpenAlex.
- Decoupling between activation time and steady-state level in input-output responses.PLoS computational biology · 2026Article
- Decoupling between activation time and steady-state level in input-output responses.bioRxiv : the preprint server for biology · 2025Article
- S100A2 promotes clear cell renal cell carcinoma tumor metastasis through regulating GLUT2 expression.Cell death & disease · 2025Article
- Comparative cofactor screens show the influence of transactivation domains and core promoters on the mechanisms of transcription.Nature genetics · 2024Article
- Regulatory properties of transcription factors with diverse mechanistic function.PLoS computational biology · 2024Article
- Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biology.Cell systems · 2023Article
- Regulation of Pre-mRNA Splicing: Indispensable Role of Post-Translational Modifications of Splicing Factors.Life (Basel, Switzerland) · 2023Review
- Activation domains can decouple the mean and noise of gene expression.Cell reports · 2022Article
- HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation.Vaccines · 2021Article
- Article
- Inhibition of spumavirus gene expression by PHF11.PLoS pathogens · 2020Article
- Determinants of transcription factor regulatory range.Nature communications · 2020Article
- A 50 year history of technologies that drove discovery in eukaryotic transcription regulation.Nature structural & molecular biology · 2019Review
- Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives.Microbial cell factories · 2019Review
- A LysR-Type Transcriptional Regulator LcrX Is Involved in Virulence, Biofilm Formation, Swimming Motility, Siderophore Secretion, and Growth in Sugar Sources inFrontiers in plant science · 2019Article
- The "readers" of unacetylated p53 represent a new class of acidic domain proteins.Nucleus (Austin, Tex.) · 2017Article
- Mechanisms of Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity.BMC genomics · 2017Article
- Systematic dissection of genomic features determining transcription factor binding and enhancer function.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Combinatorial Gene Regulation through Kinetic Control of the Transcription Cycle.Cell systems · 2017Article
- Upregulating endogenous genes by an RNA-programmable artificial transactivator.Nucleic acids research · 2015Article
70 more citing papers are in PubMed but not listed here.
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6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human immunodeficiency virus type 1 Tat fused to a DNA binding domain stimulated predominantly elongation (type IIA); and VP16, p53, and E2F1 stimulated both initiation and elongation (type IIB). A quadruple point mutation of VP16 converted it from a type IIB to a type I activator. Type I and type IIA activators synergized with one another but not with type IIB activators. This observation implies that synergy can result from the concerted action of factors stimulating two different steps in transcription: initiation and elongation. The functional differences between activators may be explained by the different contacts they make with general transcription factors. In support of this idea, we found a correlation between the abilities of activators, including Tat, to stimulate elongation and their abilities to bind TFIIH.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.