ArticleMolecular and cellular biology1996
Chromatin remodeling during Saccharomyces cerevisiae ADH2 gene activation.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 94 citations in OpenAlex.
- Article
- Genetic circuit design automation for yeast.Nature microbiology · 2020Article
- Promoter Architecture and Promoter Engineering inMetabolites · 2020Review
- Functional complementation of sir2Δ yeast mutation by the human orthologous gene SIRT1.PloS one · 2013Article
- H4K16 acetylation affects recombination and ncRNA transcription at rDNA in Saccharomyces cerevisiae.Molecular biology of the cell · 2012Article
- Snf1/AMPK regulates Gcn5 occupancy, H3 acetylation and chromatin remodelling at S. cerevisiae ADY2 promoter.Biochimica et biophysica acta · 2012Article
- Activation-induced disruption of nucleosome position clusters on the coding regions of Gcn4-dependent genes extends into neighbouring genes.Nucleic acids research · 2011Article
- Activator-independent transcription of Snf1-dependent genes in mutants lacking histone tails.Molecular microbiology · 2011Article
- The DNA sequence-dependence of nucleosome positioning in vivo and in vitro.Journal of biomolecular structure & dynamics · 2010Article
- RNA polymerase I transcription silences noncoding RNAs at the ribosomal DNA locus in Saccharomyces cerevisiae.Eukaryotic cell · 2010Article
- A translational signature for nucleosome positioning in vivo.Nucleic acids research · 2009Article
- Chromatin-dependent transcription factor accessibility rather than nucleosome remodeling predominates during global transcriptional restructuring in Saccharomyces cerevisiae.Molecular biology of the cell · 2009Article
- Poly(dA:dT) tracts: major determinants of nucleosome organization.Current opinion in structural biology · 2009Review
- The transcriptional coactivators SAGA, SWI/SNF, and mediator make distinct contributions to activation of glucose-repressed genes.The Journal of biological chemistry · 2008Article
- Dispersed mutations in histone H3 that affect transcriptional repression and chromatin structure of the CHA1 promoter in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases.Nucleic acids research · 2008Article
- Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression.Molecular and cellular biology · 2008Article
- The roles of Rad16 and Rad26 in repairing repressed and actively transcribed genes in yeast.DNA repair · 2007Article
- Chromatin disassembly from the PHO5 promoter is essential for the recruitment of the general transcription machinery and coactivators.Molecular and cellular biology · 2007Article
- A common topology for bacterial and eukaryotic transcription initiation?EMBO reports · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analyzed at both low and high resolution the distribution of nucleosomes over the Saccharomyces cerevisiae ADH2 promoter region in its chromosomal location, both under repressing (high-glucose) conditions and during derepression. Enzymatic treatments (micrococcal nuclease and restriction endonucleases) were used to probe the in vivo chromatin structure during ADH2 gene activation. Under glucose-repressed conditions, the ADH2 promoter was bound by a precise array of nucleosomes, the principal ones positioned at the RNA initiation sites (nucleosome +1), at the TATA box (nucleosome -1), and upstream of the ADR1-binding site (UAS1) (nucleosome -2). The UAS1 sequence and the adjacent UAS2 sequence constituted a nucleosome-free region. Nucleosomes -1 and +1 were destabilized soon after depletion of glucose and had become so before the appearance of ADH2 mRNA. When the transcription rate was high, nucleosomes -2 and +2 also underwent rearrangement. When spheroplasts were prepared from cells grown in minimal medium, detection of this chromatin remodeling required the addition of a small amount of glucose. Cells lacking the ADR1 protein did not display any of these chromatin modifications upon glucose depletion. Since the UAS1 sequence to which Adr1p binds is located immediately upstream of nucleosome -1, Adr1p is presumably required for destabilization of this nucleosome and for aiding the TATA-box accessibility to the transcription machinery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.