ArticleMolecular & general genetics : MGG1996
Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 64 citations in OpenAlex.
- Dual transcriptome ofJournal of oral microbiology · 2023Article
- Overexpression approaches to advance understanding of Candida albicans.Molecular microbiology · 2022Review
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Recent Advances in Genome Editing Tools in Medical Mycology Research.Journal of fungi (Basel, Switzerland) · 2021Review
- Phosphorylation regulates polarisation of chitin synthesis in Candida albicans.Journal of cell science · 2010Article
- The Candida albicans pescadillo homolog is required for normal hypha-to-yeast morphogenesis and yeast proliferation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments.Current genetics · 2006Article
- Conserved fungal genes as potential targets for broad-spectrum antifungal drug discovery.Eukaryotic cell · 2006Article
- Analysis of the Candida albicans Als2p and Als4p adhesins suggests the potential for compensatory function within the Als family.Microbiology (Reading, England) · 2005Article
- Disruption of the Candida albicans CYB5 gene results in increased azole sensitivity.Antimicrobial agents and chemotherapy · 2004Article
- Shuttle vectors for Candida albicans: control of plasmid copy number and elevated expression of cloned genes.Current genetics · 2004Article
- Molecular genetic and genomic approaches to the study of medically important fungi.Infection and immunity · 2003Review
- Candida albicans sterol C-14 reductase, encoded by the ERG24 gene, as a potential antifungal target site.Antimicrobial agents and chemotherapy · 2002Article
- Phenotypic switching in Candida albicans is controlled by a SIR2 gene.The EMBO journal · 1999Article
- Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue.The Journal of cell biology · 1999Article
- Rare homologous gene targeting in Histoplasma capsulatum: disruption of the URA5Hc gene by allelic replacement.Journal of bacteriology · 1998Article
- Electrotransformation and expression of bacterial genes encoding hygromycin phosphotransferase and beta-galactosidase in the pathogenic fungus Histoplasma capsulatum.Infection and immunity · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To allow the regulated expression of cloned genes in Candida albicans, a plasmid was constructed using the inducible promoter of the C. Albicans MAL2 gene. To demonstrate that the MAL2 promoter could regulate cloned genes placed under its control, a fusion construct was made with the coding sequence of the C. albicans URA3 gene. This plasmid was introduced into a Ura- strain of C. albicans using the process of restriction enzyme-mediated integration (REMI). This procedure involves the transformation of the BamHI-linearized plasmid in the presence of BamHI enzyme. The majority of transformants generated contained insertions of the plasmid at chromosomal BamHI sites. All transformants examined were inducible for URA3 expression, which was determined by growth analysis and by measuring the level of URA3 gene product activity. The URA+ phenotype of the transformants was stable during growth under nonselective conditions. This system offers the advantages of stable transformation, easy recovery of integrated DNA, and inducible expression of genes in C. albicans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.