ArticleMolecular & general genetics : MGG1996
The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed.
- Genome editing in the green alga Chlamydomonas: past, present practice and future prospects.The Plant journal : for cell and molecular biology · 2025Review
- Distribution and bulk flow analyses of the intraflagellar transport (IFT) motor kinesin-2 support an "on-demand" model for Chlamydomonas ciliary length control.Cytoskeleton (Hoboken, N.J.) · 2024Article
- The synthetic future of algal genomes.Cell genomics · 2024Review
- Current Nuclear Engineering Strategies in the Green MicroalgaLife (Basel, Switzerland) · 2023Review
- Article
- Advanced pathway engineering for phototrophic putrescine production.Plant biotechnology journal · 2022Article
- New destination vectors facilitate Modular Cloning for Chlamydomonas.Current genetics · 2022Article
- Molecular Advancements Establishing Chlamydomonas as a Host for Biotechnological Exploitation.Frontiers in plant science · 2022Review
- A novel, robust and mating-competentBiotechnology reports (Amsterdam, Netherlands) · 2021Article
- Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae.Microbial cell factories · 2020Article
- CrABCA2 Facilitates Triacylglycerol Accumulation inMolecules and cells · 2020Article
- Blasticidin S Deaminase: A New Efficient Selectable Marker forFrontiers in plant science · 2020Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- Light-dependent N-terminal phosphorylation of LHCSR3 and LHCB4 are interlinked in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- miRNA-Mediated Regulation of Synthetic Gene Circuits in the Green Alga Chlamydomonas reinhardtii.ACS synthetic biology · 2019Article
- Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus.BMC biotechnology · 2019Article
- Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression inPlant methods · 2019Article
- Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas.The Plant cell · 2018Article
- Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
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Abstract
A chimeric gene composed of the coding sequence of the ble gene from Streptoalloteichus hindustanus fused to the 5' and 3' untranslated regions of the Chlamydomonas reinhardtii nuclear gene RBCS2 has been constructed. Introduction of this chimeric gene into the nuclear genome of C. reinhardtii by co-transformation with the ARG7 marker yields Arg+ transformants of which approximately 80% possess the ble gene. Of these co-transformants, approximately 3% display a phleomycin-resistant (PmR) phenotype. Western blot analysis using antibodies against the ble gene product confirms the presence of the protein in the PmR transformants and genetic analysis demonstrates the co-segregation of the ble gene with the phenotype in progeny arising from the mating of a PmR transformant to wild-type strains. Direct selection of PmR transformants was achieved by allowing an 18-h period for recovery and growth of transformed cells prior to selection. This work represents the first demonstration of stable expression and inheritance of a foreign gene in the nuclear genome of C. reinhardtii and provides a useful dominant marker for nuclear transformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.