ArticleMolecular & general genetics : MGG1996
Hordein promoter methylation and transcriptional activity in wild-type and mutant barley endosperm.
Article in Molecular & general genetics : MGG, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- Characterization of barley (Horduem vulgare) lys3 mutants identifies genes under the regulation of the prolamin-box binding transcription factor and elucidates its role in endosperm promoter methylation during grain development.Molecular genetics and genomics : MGG · 2024Article
- Targeting Induced Local Lesions in the WheatFrontiers in nutrition · 2022Article
- Omics Data Reveal Putative Regulators of Einkorn Grain Protein Composition under Sulfur Deficiency.Plant physiology · 2020Article
- Development of Decreased-Gluten Wheat Enabled by Determination of the Genetic Basis ofPlant physiology · 2019Article
- Transgenic expression of phytase in wheat endosperm increases bioavailability of iron and zinc in grains.Transgenic research · 2017Article
- Reconstruction of the astaxanthin biosynthesis pathway in rice endosperm reveals a metabolic bottleneck at the level of endogenous β-carotene hydroxylase activity.Transgenic research · 2017Article
- Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid.Transgenic research · 2016Article
- Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor-product balance.Plant biotechnology journal · 2016Article
- Alterations of histone modifications at the senescence-associated gene HvS40 in barley during senescence.Plant molecular biology · 2015Article
- Combined transcript, proteome, and metabolite analysis of transgenic maize seeds engineered for enhanced carotenoid synthesis reveals pleotropic effects in core metabolism.Journal of experimental botany · 2015Article
- Conserved cis-regulatory modules in promoters of genes encoding wheat high-molecular-weight glutenin subunits.Frontiers in plant science · 2014Article
- Article
- Structural genes of wheat and barley 5-methylcytosine DNA glycosylases and their potential applications for human health.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Tissue-specific differences in cytosine methylation and their association with differential gene expression in sorghum.Plant physiology · 2011Article
- Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants.Plant cell reports · 2009Article
- Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
- Segmental distribution of genes harboring a CpG island-like region on rice chromosomes.Molecular genetics and genomics : MGG · 2006Article
- The methylation cycle and its possible functions in barley endosperm development.Plant molecular biology · 2005Article
- Computational approaches to identify promoters and cis-regulatory elements in plant genomes.Plant physiology · 2003Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
B- and C-hordein gene transcription is severely reduced in the endosperm of the regulatory barley mutant lys3a, and this is correlated with persistent hypermethylation of the promoters. In contrast, D-hordein is expressed at normal levels in the mutant. To confirm the connection between methylation and transcriptional activity, a genomic D-hordein clone was isolated and sequenced. The nucleotide composition of the promoter region revealed a CpG island and methylation analysis, using bisulphite treatment of genomic DNA, confirmed that the D-hordein promoter is unmethylated in endosperm and leaf tissue. Immunocytochemical studies localized D-hordein to the reticular component of protein bodies in both the wild-type Bomi and lys3a. Transient expression of GUS reporter gene constructs in barley endosperm, following transfection by particle bombardment revealed the D-hordein promotors. Comparison of transient expression in Bomi and lys3a endosperm demonstrated that the activities of the unmethylated D-hordein and the Hor1-14 C-hordein promoters were equivalent, while the activities in the mutant of the Horl-17 C-hordein and the Hor2-4 B-hordein promoters were reduced two- and tenfold, respectively. Methylation of plasmids in vitro prior to expression severely inhibited B- and D-hordein promoter activities. Based on these observations two categories of promoters for endosperm-specific expression of storage proteins are recognized and a model involving methylation and modulation of chromatin structure in the regulation by the Lys3 gene is presented.
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