ArticleJournal of virology1996
Astrovirus ribosomal frameshifting in an infection-transfection transient expression system.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Novel goose-origin astrovirus infection in geese: the effect of age at infection.Poultry science · 2020Article
- Propagation of Astrovirus VA1, a Neurotropic Human Astrovirus, in Cell Culture.Journal of virology · 2017Article
- Crystal Structure of the Human Astrovirus Capsid Protein.Journal of virology · 2016Article
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- Complete genome sequence of a duck astrovirus discovered in eastern China.Journal of virology · 2012Article
- Animal virus schemes for translation dominance.Current opinion in virology · 2011Review
- Astrovirus infections in humans and animals - molecular biology, genetic diversity, and interspecies transmissions.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2011Review
- Crystal structure of the human astrovirus capsid spike.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Detection of a novel astrovirus in brain tissue of mink suffering from shaking mink syndrome by use of viral metagenomics.Journal of clinical microbiology · 2010Article
- Complete genome sequence of human astrovirus genotype 6.Virology journal · 2010Article
- Virus versus host cell translation love and hate stories.Advances in virus research · 2009Review
- Novel astroviruses in insectivorous bats.Journal of virology · 2008Article
- Assembly of severe acute respiratory syndrome coronavirus RNA packaging signal into virus-like particles is nucleocapsid dependent.Journal of virology · 2005Article
- Identification of structural domains involved in astrovirus capsid biology.Viral immunology · 2005Review
- C-terminal nsP1a protein of human astrovirus colocalizes with the endoplasmic reticulum and viral RNA.Journal of virology · 2004Article
- Structural requirements of astrovirus virus-like particles assembled in insect cells.Journal of virology · 2004Article
- Identification of a novel protein 3a from severe acute respiratory syndrome coronavirus.FEBS letters · 2004Article
- Protein products of the open reading frames encoding nonstructural proteins of human astrovirus serotype 8.Journal of virology · 2003Article
- V, 2.Ribosomal frameshifting in astroviruses.Perspectives in medical virology · 2003Article
- Astroviruses.Perspectives in medical virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Different regions of the human astrovirus frameshift signal were cloned into the rhesus rotavirus VP4 gene and evaluated in an infection-transfection transient expression cell culture system. BHK-21 cells, infected with a vaccinia virus that expresses T7 RNA polymerase (vTF7-3), were transfected with the various astrovirus-VP4 constructs. All constructs were driven by a T7 promoter and contained an internal ribosome entry site. Frameshifted and nonframeshifted protein products were immunoprecipitated with VP4 amino- and carboxy-terminal-specific monoclonal antibodies, and their ratios were determined by PhosphorImager analysis. The efficiency of frameshifting was 25 to 28%, significantly greater than the 5 to 7% efficiency reported previously in a cell-free translation system. Coupling of transcription and translation in a cell-free system yielded frameshifting efficiencies threefold greater than that of the uncoupled in vitro system. The presence of the shifty heptamer was an absolute requirement for frameshifting in both cell-free and intact-cell systems, while deletion of the potential downstream pseudoknot region did not affect the efficiency of frameshifting.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.