ArticleJournal of virology1996
A preferred region for recombinational patch repair in the 5' untranslated region of primer binding site-impaired murine leukemia virus vectors.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Identification of a putative Gag binding site critical for feline immunodeficiency virus genomic RNA packaging.RNA (New York, N.Y.) · 2023Article
- Recombination is required for efficient HIV-1 replication and the maintenance of viral genome integrity.Nucleic acids research · 2018Article
- Structural basis of genomic RNA (gRNA) dimerization and packaging determinants of mouse mammary tumor virus (MMTV).Retrovirology · 2014Article
- Cross-packaging of genetically distinct mouse and primate retroviral RNAs.Retrovirology · 2009Article
- Genetic recombination between human immunodeficiency virus type 1 (HIV-1) and HIV-2, two distinct human lentiviruses.Journal of virology · 2008Article
- Analysis of the contribution of reverse transcriptase and integrase proteins to retroviral RNA dimer conformation.Journal of virology · 2005Article
- Complementarity-directed RNA dimer-linkage promotes retroviral recombination in vivo.Nucleic acids research · 2004Article
- Template dimerization promotes an acceptor invasion-induced transfer mechanism during human immunodeficiency virus type 1 minus-strand synthesis.Journal of virology · 2003Article
- Dimerization and template switching in the 5' untranslated region between various subtypes of human immunodeficiency virus type 1.Journal of virology · 2003Article
- Effect of the murine leukemia virus extended packaging signal on the rates and locations of retroviral recombination.Journal of virology · 2000Article
- Selection of functional tRNA primers and primer binding site sequences from a retroviral combinatorial library: identification of new functional tRNA primers in murine leukemia virus replication.Nucleic acids research · 2000Article
- Mutations of the kissing-loop dimerization sequence influence the site specificity of murine leukemia virus recombination in vivo.Journal of virology · 2000Article
- The kissing-loop motif is a preferred site of 5' leader recombination during replication of SL3-3 murine leukemia viruses in mice.Journal of virology · 1999Article
- Effect of distance between homologous sequences and 3' homology on the frequency of retroviral reverse transcriptase template switching.Journal of virology · 1999Article
- Replication and pathogenicity of primer binding site mutants of SL3-3 murine leukemia viruses.Journal of virology · 1999Article
- Recombination in the 5' leader of murine leukemia virus is accurate and influenced by sequence identity with a strong bias toward the kissing-loop dimerization region.Journal of virology · 1998Article
- Extended minus-strand DNA as template for R-U5-mediated second-strand transfer in recombinational rescue of primer binding site-modified retroviral vectors.Journal of virology · 1998Article
- Correlated template-switching events during minus-strand DNA synthesis: a mechanism for high negative interference during retroviral recombination.Journal of virology · 1998Article
- Psi- vectors: murine leukemia virus-based self-inactivating and self-activating retroviral vectors.Journal of virology · 1997Article
- Mutations in the kissing-loop hairpin of human immunodeficiency virus type 1 reduce viral infectivity as well as genomic RNA packaging and dimerization.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transduction of primer binding site-impaired Akv murine leukemia virus-based retroviral vectors from the murine packaging cell lines psi-2 and omega E was studied. The efficiency of transduction of the neo marker of all mutated constructs was found to decrease by 5 to 6 orders of magnitude compared with that of the wild-type vector. Thirty-two of 60 transduced proviruses analyzed harbored a primer binding site sequence matching a glutamine tRNA primer. Sequence analysis of the regions flanking the glutamine tRNA primer binding site revealed a distinct pattern of nucleotide differences from the Akv-based vector, suggesting the involvement of a specific endogenous virus-like sequence in patch repair rescue of the primer binding site mutants. The putative recombination partner RNA was found in virions from psi-2 cells as detected by analysis of glutamine tRNA-initiated cDNA and by sequence analysis of regions at or around the glutamine tRNA primer binding site. We propose that the forced recombination of primer binding site mutants involves initial priming on endogenous viral sequences and requires template switching during minus-strand synthesis in the region between the neo gene and the mutated primer binding site to allow correct second-strand transfer in reverse transcription. The system thereby selects for a reverse transcriptase-mediated recombination event in the 5' untranslated region. A panel of sequence differences between the recombination partners in this region has allowed mapping of the site of recombination for each transduction event. Interestingly, the majority of the recombination events were clustered within a narrow, 33-nucleotide region though to be involved in genomic RNA dimerization.
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