ArticleJournal of virology1996
Human cytomegalovirus carries serine/threonine protein phosphatases PP1 and a host-cell derived PP2A.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Protein Phosphatase 1 Regulates Human Cytomegalovirus Protein Translation by Restraining AMPK Signaling.Frontiers in microbiology · 2021Article
- Mass Spectrometry-Based Characterization of the Virion Proteome, Phosphoproteome, and Associated Kinase Activity of Human Cytomegalovirus.Microorganisms · 2020Review
- HSC70 is required for infectious bursal disease virus (IBDV) infection in DF-1 cells.Virology journal · 2020Article
- Human cytomegalovirus phosphoproteins are hypophosphorylated and intrinsically disordered.The Journal of general virology · 2017Article
- The tegument protein pp65 of human cytomegalovirus acts as an optional scaffold protein that optimizes protein uploading into viral particles.Journal of virology · 2014Article
- Review
- Article
- Human cytomegalovirus tegument protein pp150 acts as a cyclin A2-CDK-dependent sensor of the host cell cycle and differentiation state.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Synthesis, transport and antiviral activity of Ala-Ser and Val-Ser prodrugs of cidofovir.Bioorganic & medicinal chemistry letters · 2011Article
- Identification of replication-competent HSV-1 Cgal+ strain signaling targets in human hepatoma cells by functional organelle proteomics.Molecular & cellular proteomics : MCP · 2009Article
- Cellular serine/threonine phosphatase activity during human cytomegalovirus infection.Virology · 2008Article
- Article
- Dephosphorylation of JC virus agnoprotein by protein phosphatase 2A: inhibition by small t antigen.Virology · 2008Article
- Small tumor antigen of polyomaviruses: role in viral life cycle and cell transformation.Journal of cellular physiology · 2008Review
- Application of proteomics technology for analyzing the interactions between host cells and intracellular infectious agents.Proteomics · 2008Review
- Human herpesvirus 6 open reading frame U14 protein and cellular p53 interact with each other and are contained in the virion.Journal of virology · 2005Article
- Plunder and stowaways: incorporation of cellular proteins by enveloped viruses.Journal of virology · 2005Review
- Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome.Journal of virology · 2004Article
- Human cytomegalovirus carries a cell-derived phospholipase A2 required for infectivity.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human cytomegalovirus (CMV), a herpesvirus, is an important cause of morbidity and mortality in immunocompromised patients. When studying hyper-immediate-early events after contact between CMV virions and the cell membrane, we observed a hypophosphorylation of cellular proteins within 10 min. This can be explained in part by our finding that purified CMV contains serine/threonine protein phosphatase activities. Biochemical analyses indicate that this protein phosphatase activity has all characteristics of type 1 and 2A protein phosphatases (PP1 and PP2A). Specifically, PP1 accounts for approximately 30% and PP2A accounts for the remaining 70% of the phosphorylase phosphatase activity found. CMV produced in astrocytoma cells stably expressing an amino-terminally tagged PP2A catalytic subunit contained tagged enzyme, thus demonstrating the cellular origin of CMV-associated PP2A. PP2A is specifically found inside the virus, associated with the nucleocapsid fraction. Western blot (immunoblot) analysis of purified virus revealed the presence of the catalytic subunits of PP2A and PP1. Furthermore, the catalytic subunit of PP2A appears to be complexed to the regulatory subunits PR65 and PR55, which is also the most abundant configuration of this enzyme found in the host cells. Incubation of virus with okadaic acid before contact of CMV with cells prevented hypophosphorylation of cellular proteins, thus demonstrating the role of CMV-associated phosphatases in this phenomenon. CMV can thus transport an active enzyme from one cell to another.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.