ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1996
Microtubule stability decreases axon elongation but not axoplasm production.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 118 citations in OpenAlex.
- Effects of Epothilone D on Social Defeat Stress-induced Changes in Microtubule-related and Endoplasmic Reticulum Stress Protein Expression.Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology · 2025Article
- Actin cytoskeletal dynamics do not impose an energy drain on growth cone bioenergetics.Journal of cell science · 2023Article
- Contributions of microtubule dynamics and transport to presynaptic and postsynaptic functions.Molecular and cellular neurosciences · 2022Article
- Precision genetic cellular models identify therapies protective against ER stress.Cell death & disease · 2021Article
- With the Permission of Microtubules: An Updated Overview on Microtubule Function During Axon Pathfinding.Frontiers in molecular neuroscience · 2021Review
- Neuronal-Specific TUBB3 Is Not Required for Normal Neuronal Function but Is Essential for Timely Axon Regeneration.Cell reports · 2018Article
- Expression of acetylated tubulin in the postnatal developing mouse cochlea.European journal of histochemistry : EJH · 2018Article
- Disruption of lipid-raft localized GαNeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Neurite elongation is highly correlated with bulk forward translocation of microtubules.Scientific reports · 2017Article
- (S)-Lacosamide Binding to Collapsin Response Mediator Protein 2 (CRMP2) Regulates CaV2.2 Activity by Subverting Its Phosphorylation by Cdk5.Molecular neurobiology · 2016Article
- Nerve growth factor stimulates axon outgrowth through negative regulation of growth cone actomyosin restraint of microtubule advance.Molecular biology of the cell · 2016Article
- Dendrite and Axon Specific Geometrical Transformation in Neurite Development.Frontiers in computational neuroscience · 2015Article
- TACC3 is a microtubule plus end-tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types.Molecular biology of the cell · 2014Article
- Article
- Myosin II regulates activity dependent compensatory endocytosis at central synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- CNP/cGMP signaling regulates axon branching and growth by modulating microtubule polymerization.Developmental neurobiology · 2013Article
- Microtubule plus-end tracking proteins SLAIN1/2 and ch-TOG promote axonal development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Regulation of mitochondrial transport and inter-microtubule spacing by tau phosphorylation at the sites hyperphosphorylated in Alzheimer's disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- KBP interacts with SCG10, linking Goldberg-Shprintzen syndrome to microtubule dynamics and neuronal differentiation.Human molecular genetics · 2010Article
- Cytosolic G{alpha}s acts as an intracellular messenger to increase microtubule dynamics and promote neurite outgrowth.The Journal of biological chemistry · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules are a primary cytoskeletal constituent of axons and growth cones. In addition to serving as a scaffolding for axon assembly, they also provide a means of transport of organelles that are essential for outgrowth and maintenance of synaptic function. Pharmacological manipulations that disrupt net assembly of microtubules also interfere with growth cone advance and axon extension. Less is known after the effects of disrupting microtubule dynamics without affecting net assembly. To investigate this, we studied the effects of low doses of nocodazole on axon extension and microtubule organization in rat superior cervical ganglion neurons. We report that 165-330 nM nocodazole significantly reduces axon extension rate and increases axon diameter without affecting the rate of production of axoplasm or microtubule polymer, and without decreasing the average length or number of microtubules. Two observations suggested that microtubule dynamics were depressed by this dose of nocodazole. First, this treatment eliminated the highly divergent lengths and positions of microtubules characteristic of normal growth cones, inducing an array in which each microtubule terminated at roughly the same position in the proximal regions of the growth cone. Second, there was a decrease in the proportion of microtubule length containing mostly tyrosinated (newly assembled) alpha-tubulin and an increase in the proportion of microtubule length containing mostly acetylated (older, more stable) alpha-tubulin. Together, these data suggest that a decrease in dynamic instability of microtubules is sufficient to disrupt axon extension but does not interfere with axoplasm production.
Indexed as
Identifiers
8627361PMC6579152W1894865050What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.