ArticleJournal of bacteriology1996
Identification and characterization of the eps (Exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 325 citations in OpenAlex.
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- Flavour by design: food-grade lactic acid bacteria improve the volatile aroma spectrum of oat milk, sunflower seed milk, pea milk, and faba milk towards improved flavour and sensory perception.Microbial cell factories · 2023Article
- Bacteriophage-host interactions in Streptococcus thermophilus and their impact on co-evolutionary processes.FEMS microbiology reviews · 2023Review
- In Vitro Assessment of Lactobacillus crispatus UBLCp01, Lactobacillus gasseri UBLG36, and Lactobacillus johnsonii UBLJ01 as a Potential Vaginal Probiotic Candidate.Probiotics and antimicrobial proteins · 2023Article
- Integrating metagenomic binning with flux balance analysis to unravel syntrophies in anaerobic COMicrobiome · 2022Article
- In vitro screening of EPS-producingFood science & nutrition · 2022Article
- Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses.Archives of microbiology · 2021Article
- Comparative Genomics and Specific Functional Characteristics Analysis ofMicroorganisms · 2021Article
- Characterization of Lactic Acid Bacteria in Raw Buffalo Milk: a Screening for Novel Probiotic Candidates and Their Transcriptional Response to Acid Stress.Probiotics and antimicrobial proteins · 2021Article
- Response of Lactobacillus plantarum VAL6 to challenges of pH and sodium chloride stresses.Scientific reports · 2021Article
- Exploring the Role of Bacterial Extracellular Polymeric Substances for Sustainable Development in Agriculture.Current microbiology · 2020Review
- Genetic and Phenotypic Characteristics of a Multi-strain Probiotic for Broilers.Current microbiology · 2020Article
- Relationship Between PutativeFrontiers in microbiology · 2018Article
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- Predictable Molecular Adaptation of CoevolvingFrontiers in microbiology · 2018Article
- Molecular Cloning, Expression and Characterization of Oenococcus oeni Priming Glycosyltransferases.Molecular biotechnology · 2017Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the identification and characterization of the eps gene cluster of Streptococcus thermophilus Sfi6 required for exopolysaccharide (EPS) synthesis. This report is the first genetic work concerning EPS production in a food microorganism. The EPS secreted by this strain consists of the following tetrasaccharide repeating unit:-->3)-beta-D-Galp-(1-->3)-[alpha-D-Galp-(1-->6)]-beta-D- D-Galp-(1-->3)-alpha-D-Galp-D-GalpNAc-(1-->. The genetic locus The genetic locus was identified by Tn916 mutagenesis in combination with a plate assay to identify Eps mutants. Sequence analysis of the gene region, which was obtained from subclones of a genomic library of Sfi6, revealed a 15.25-kb region encoding 15 open reading frames. EPS expression in the non-EPS-producing heterologous host, Lactococcus lactis MG1363, showed that within the 15.25-kb region, a region with a size of 14.52 kb encoding the 13 genes epsA to epsM was capable of directing EPS synthesis and secretion in this host. Homology searches of the predicted proteins in the Swiss-Prot database revealed high homology (40 to 68% identity) for epsA, B, C, D, and E and the genes involved in capsule synthesis in Streptococcus pneumoniae and Streptococcus agalactiae. Moderate to low homology (37 to 18% identity) was detected for epsB, D, F, and H and the genes involved in capsule synthesis in Staphylococcus aureus for epsC, D, and E and the genes involved in exopolysaccharide I (EPSI) synthesis in Rhizobium meliloti for epsC to epsJ and the genes involved in lipopolysaccharide synthesis in members of the Enterobacteriaceae, and finally for eps K and lipB of Neisseria meningitidis. Genes (epsJ, epsL, and epsM) for which the predicted proteins showed little or no homology with proteins in the Swiss-Prot database were shown to be involved in EPS synthesis by single-crossover gene disruption experiments.
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