ArticleThe Plant cell1996
Cloning and functional expression of a plant voltage-dependent chloride channel.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 96 citations in OpenAlex.
- Research Progress on Chloride Channel- and Transporter- Related Gene Families in Plants.International journal of molecular sciences · 2026Review
- Article
- CLC gene family inFrontiers in plant science · 2025Article
- Review
- Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the HalophytePlants (Basel, Switzerland) · 2022Article
- Genome-Wide Identification and Functional Characterization of the Chloride Channel TaCLC Gene Family in Wheat (Frontiers in genetics · 2022Article
- GmDREB6, a soybean transcription factor, notably affects the transcription of theSaudi journal of biological sciences · 2021Article
- Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its roles in salt resistance.BMC plant biology · 2020Article
- Article
- Article
- The GEF1 proton-chloride exchanger affects tombusvirus replication via regulation of copper metabolism in yeast.Journal of virology · 2013Article
- Regulation by salt of vacuolar H+-ATPase and H+-pyrophosphatase activities and Na+/H+ exchange.Plant signaling & behavior · 2009Review
- Membrane transporters and carbon metabolism implicated in chloride homeostasis differentiate salt stress responses in tolerant and sensitive Citrus rootstocks.Functional & integrative genomics · 2009Article
- Residues important for nitrate/proton coupling in plant and mammalian CLC transporters.The Journal of biological chemistry · 2009Article
- Review. CLC-mediated anion transport in plant cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009Review
- Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource.The New phytologist · 2003Review
- Oligoguluronates elicit an oxidative burst in the brown algal kelp Laminaria digitata.Plant physiology · 2001Article
- CLC-Nt1, a putative chloride channel protein of tobacco, co-localizes with mitochondrial membrane markers.The Biochemical journal · 2000Article
- Possible Involvement of Al-Induced Electrical Signals in Al Tolerance in Wheat.Plant physiology · 1997Article
- Molecular dissection of gating in the ClC-2 chloride channel.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cell membrane anion channels participate in basic physiological functions, such as cell volume regulation and signal transduction. However, nothing is known about their molecular structure. Using a polymerase chain reaction strategy, we have cloned a tobacco cDNA (CIC-Nt1) encoding a 780-amino acid protein with several putative transmembrane domains. CIC-Nt1 displays 24 to 32% amino acid identity with members of the animal voltage-dependent chloride channel (CIC) family, whose archetype is CIC-0 from the Torpedo marmorata electric organ. Injection of CIC-Nt1 complementary RNA into Xenopus oocytes elicited slowly activating inward currents upon membrane hyperpolarization more negative than -120 mV. These currents were carried mainly by anions, modulated by extracellular anions, and totally blocked by 10 mM extracellular calcium. The identification of CIC-Nt1 extends the CIC family to higher plants and provides a molecular probe for the study of voltage-dependent anion channels in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.