ArticleProceedings of the National Academy of Sciences of the United States of America1996
Targeted gene disruption of Hsp70-2 results in failed meiosis, germ cell apoptosis, and male infertility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 142 papers.
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Who cites it
142 citing papers in PubMed, 558 citations in OpenAlex.
- Use of hyaluronan in the selection of sperm for intracytoplasmic sperm injection (ICSI): significant improvement in clinical outcomes--multicenter, double-blinded and randomized controlled trial.Human reproduction (Oxford, England) · 2013Trial
- Exploratory Cross-Cohort Transcriptomic Comparison of Coronary Artery Disease and Non-Obstructive Azoospermia.International journal of molecular sciences · 2026Article
- Expression and Localization of a Testis-Specific Heat Shock Protein (CfHSP70-2) During Spermatogenesis in the Spruce Budworm, Choristoneura fumiferana (Clem.).Archives of insect biochemistry and physiology · 2026Article
- The synaptonemal complex: structure, function, and clinical implications†.Biology of reproduction · 2026Article
- Crocin Supplementation of Semen Extender Preserves Chilled Ram Semen Quality During Extended Storage.Animals : an open access journal from MDPI · 2026Article
- A Descriptive Study of Brown Bear (Animals : an open access journal from MDPI · 2025Article
- IL-28A/IL-10Rβ axis promotes angiogenesis via eNOS/AKT signaling and AP-1/NF-κB/MMP-2 network by regulating HSP70-1 expression.Journal of advanced research · 2025Article
- The asymmetric expression of HSPA2 in blastomeres governs the first embryonic cell-fate decision.eLife · 2025Article
- Dysregulation of N-glycosylation byInternational journal of biological sciences · 2025Article
- The conserved genetic program of male germ cells uncovers ancient regulators of human spermatogenesis.eLife · 2024Article
- Destabilization of mRNAs enhances competence to initiate meiosis in mouse spermatogenic cells.Development (Cambridge, England) · 2024Article
- FromiScience · 2024Article
- Impact of trypanosomiasis on male camel infertility.Frontiers in veterinary science · 2024Article
- The Application of Ejaculate-Based Shotgun Proteomics for Male Infertility Screening.Biomedicines · 2023Article
- Regulation of germline proteostasis by HSF1 and insulin/IGF-1 signaling.Biochemical Society transactions · 2023Article
- Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Article
- Expression Analysis of Molecular Chaperones Hsp70 and Hsp90 on Development and Metabolism of Different Organs and Testis in Cattle (Cattle-yak and Yak).Metabolites · 2022Article
- Heat shock protein 101 contributes to the thermotolerance of male meiosis in maize.The Plant cell · 2022Article
- Protecting the future: balancing proteostasis for reproduction.Trends in cell biology · 2022Review
82 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In addition to the five 70-kDa heat shock proteins (HSP70) common to germ cells and somatic tissues of mammals, spermatogenic cells synthesize HSP70-2 during meiosis. To determine if this unique stress protein has a critical role in meiosis, we used gene-targeting techniques to disrupt Hsp70-2 in mice. Male mice homozygous for the mutant allele (Hsp70-2 -/-) did not synthesize HSP70-2, lacked postmeiotic spermatids and mature sperm, and were infertile. However, neither meiosis nor fertility was affected in female Hsp70-2 -/- mice. We previously found that HSP70-2 is associated with synaptonemal complexes in the nucleus of meiotic spermatocytes from mice and hamsters. While synaptonemal complexes assembled in Hsp70-2 -/- spermatocytes, structural abnormalities became apparent in these cells by late prophase, and development rarely progressed to the meiotic divisions. Furthermore, analysis of nuclei and genomic DNA indicated that the failure of meiosis in Hsp70-2 -/- mice was coincident with a dramatic increase in spermatocyte apoptosis. These results suggest that HSP70-2 participates in synaptonemal complex function during meiosis in male germ cells and is linked to mechanisms that inhibit apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.