ArticleProceedings of the National Academy of Sciences of the United States of America1996
Mercuric ion reduction and resistance in transgenic Arabidopsis thaliana plants expressing a modified bacterial merA gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 423 citations in OpenAlex.
- Methylmercury demethylation and volatilization by animals expressing microbial enzymes.Nature communications · 2025Article
- Improving NiInternational journal of molecular sciences · 2024Article
- Evaluating the Accumulation of Grain Mercury in Engineered Rice Lines ContainingPlants (Basel, Switzerland) · 2024Article
- Helping plants to deal with heavy metal stress: the role of nanotechnology and plant growth promoting rhizobacteria in the process of phytoremediation.Environmental science and pollution research international · 2022Review
- Is Genetic Engineering a Route to Enhance Microalgae-Mediated Bioremediation of Heavy Metal-Containing Effluents?Molecules (Basel, Switzerland) · 2022Review
- Phytoremediation and Microorganisms-Assisted Phytoremediation of Mercury-Contaminated Soils: Challenges and Perspectives.International journal of environmental research and public health · 2021Review
- Transgenic merA and merB expression reduces mercury contamination in vegetables and grains grown in mercury-contaminated soil.Plant cell reports · 2020Article
- Bioremediation of toxic heavy metals (THMs) contaminated sites: concepts, applications and challenges.Environmental science and pollution research international · 2020Review
- Agricultural soils a trigger to nitrous oxide: a persuasive greenhouse gas and its management.Environmental monitoring and assessment · 2020Review
- Phytoextraction of Heavy Metals: A Promising Tool for Clean-Up of Polluted Environment?Frontiers in plant science · 2018Review
- Genome-wide association analysis identifies loci governing mercury accumulation in maize.Scientific reports · 2017Article
- Structural and Biochemical Characterization of Organotin and Organolead Compounds Binding to the Organomercurial Lyase MerB Provide New Insights into Its Mechanism of Carbon-Metal Bond Cleavage.Journal of the American Chemical Society · 2017Article
- Potential Biotechnological Strategies for the Cleanup of Heavy Metals and Metalloids.Frontiers in plant science · 2016Review
- Adaptive Engineering of Phytochelatin-based Heavy Metal Tolerance.The Journal of biological chemistry · 2015Article
- In situ remediation technologies for mercury-contaminated soil.Environmental science and pollution research international · 2015Review
- Pollution due to hazardous glass waste.Environmental science and pollution research international · 2014Review
- Quantitative trait loci for mercury accumulation in maize (Zea mays L.) identified using a RIL population.PloS one · 2014Article
- Article
- Metallothionein expression in chloroplasts enhances mercury accumulation and phytoremediation capability.Plant biotechnology journal · 2011Article
- Genetic engineering to enhance mercury phytoremediation.Current opinion in biotechnology · 2009Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With global heavy metal contamination increasing, plants that can process heavy metals might provide efficient and ecologically sound approaches to sequestration and removal. Mercuric ion reductase, MerA, converts toxic Hg2+ to the less toxic, relatively inert metallic mercury (Hg0) The bacterial merA sequence is rich in CpG dinucleotides and has a highly skewed codon usage, both of which are particularly unfavorable to efficient expression in plants. We constructed a mutagenized merA sequence, merApe9, modifying the flanking region and 9% of the coding region and placing this sequence under control of plant regulatory elements. Transgenic Arabidopsis thaliana seeds expressing merApe9 germinated, and these seedlings grew, flowered, and set seed on medium containing HgCl2 concentrations of 25-100 microM (5-20 ppm), levels toxic to several controls. Transgenic merApe9 seedlings evolved considerable amounts of Hg0 relative to control plants. The rate of mercury evolution and the level of resistance were proportional to the steady-state mRNA level, confirming that resistance was due to expression of the MerApe9 enzyme. Plants and bacteria expressing merApe9 were also resistant to toxic levels of Au3+. These and other data suggest that there are potentially viable molecular genetic approaches to the phytoremediation of metal ion pollution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.