ArticleMolecular and cellular biology1996
Mapping of a replication origin within the promoter region of two unlinked, abundantly transcribed actin genes of Physarum polycephalum.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Replication forks reverse at high frequency upon replication stress in Physarum polycephalum.Chromosoma · 2014Article
- RNase-dependent discontinuities associated with the crossovers of spontaneously formed joint DNA molecules in Physarum polycephalum.Chromosoma · 2010Article
- A revisionist replicon model for higher eukaryotic genomes.Journal of cellular biochemistry · 2008Review
- DNA elements modulating the KARS12 chromosomal replicator in Kluyveromyces lactis.Molecular genetics and genomics : MGG · 2007Article
- Low rate of replication fork progression lengthens the replication timing of a locus containing an early firing origin.Nucleic acids research · 2007Article
- The ribosomal RNA gene promoter and adjacent cis-acting DNA sequences govern plasmid DNA partitioning and stable inheritance in the parasitic protozoan Leishmania.Nucleic acids research · 2004Article
- Developmentally regulated usage of Physarum DNA replication origins.EMBO reports · 2003Article
- Replicational organization of three weakly expressed loci in Physarum polycephalum.Nucleic acids research · 2002Article
- Replication intermediate analysis confirms that chromosomal replication origin initiates from an unusual intergenic region in Caulobacter crescentus.Nucleic acids research · 2001Article
- Activation of silent replication origins at autonomously replicating sequence elements near the HML locus in budding yeast.Molecular and cellular biology · 1999Article
- The one-kilobase DNA fragment upstream of the ardC actin gene of Physarum polycephalum is both a replicator and a promoter.Molecular and cellular biology · 1999Article
- Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica.Molecular biology of the cell · 1999Article
- An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica.Molecular and cellular biology · 1997Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analyzed the replication of two unlinked actin genes, ardB and ardC , which are abundantly transcribed in the naturally synchronous plasmodium of the slime mold Physarum polycephalum. Detection and size measurements of single-stranded nascent replication intermediates (RIs) demonstrate that these two genes are concomitantly replicated at the onset of the 3-h S phase and tightly linked to replication origins. Appearance of RIs on neutral-neutral two-dimensional gels at specific time points in early S phase and analysis of their structure confirmed these results and further established that, in both cases, an efficient, site-specific, bidirectional origin of replication is localized within the promoter region of the gene. We also determined similar elongation rates for the divergent replication forks of the ardC gene replicon. Finally, taking advantage of a restriction fragment length polymorphism, we studied allelic replicons and demonstrate similar localizations and a simultaneous firing of allelic replication origins. Computer search revealed a low level of homology between the promoters of ardB and ardC and, most notably, the absence of DNA sequences similar to the yeast autonomously replicating sequence consensus sequence in these Physarum origin regions. Our results with the ardB and ardC actin genes support the model of early replicating origins located within the promoter regions of abundantly transcribed genes in P. polycephalum.
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