ArticleMolecular and cellular biology1996
Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Strategies to Expand the Genetic Code of Mammalian Cells.Chemical reviews · 2025Review
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Efficient Amber SuppressionACS synthetic biology · 2022Article
- A rationally designed orthogonal synthetase for genetically encoded fluorescent amino acids.Heliyon · 2020Article
- Incorporation of nonstandard amino acids into proteins: principles and applications.World journal of microbiology & biotechnology · 2020Review
- Translational Control using an Expanded Genetic Code.International journal of molecular sciences · 2019Review
- Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells.Nucleic acids research · 2018Article
- Article
- The central role of tRNA in genetic code expansion.Biochimica et biophysica acta. General subjects · 2017Review
- Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis.Journal of the American Chemical Society · 2016Article
- Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells.PloS one · 2010Article
- Genetic encoding of non-natural amino acids in Drosophila melanogaster Schneider 2 cells.Protein science : a publication of the Protein Society · 2010Article
- Expanding the genetic code for biological studies.Chemistry & biology · 2009Review
- Human Gene and Protein Database (HGPD): a novel database presenting a large quantity of experiment-based results in human proteomics.Nucleic acids research · 2009Article
- The many applications of acid urea polyacrylamide gel electrophoresis to studies of tRNAs and aminoacyl-tRNA synthetases.Methods (San Diego, Calif.) · 2008Article
- Genetically encoding unnatural amino acids for cellular and neuronal studies.Nature neuroscience · 2007Article
- Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- ORFDB: an information resource linking scientific content to a high-quality Open Reading Frame (ORF) collection.Nucleic acids research · 2004Article
- Complete set of orthogonal 21st aminoacyl-tRNA synthetase-amber, ochre and opal suppressor tRNA pairs: concomitant suppression of three different termination codons in an mRNA in mammalian cells.Nucleic acids research · 2004Article
- Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
As an approach to inducible suppression of nonsense mutations in mammalian and in higher eukaryotic cells, we have analyzed the expression of an Escherichia coli glutamine-inserting amber suppressor tRNA gene in COS-1 and CV-1 monkey kidney cells. The tRNA gene used has the suppressor tRNA coding sequence flanked by sequences derived from a human initiator methionine tRNA gene and has two changes in the coding sequence. This tRNA gene is transcribed, and the transcript is processed to yield the mature tRNA in COS-1 and CV-1 cells. We show that the tRNA is not aminoacylated in COS-1 cells by any of the endogenous aminoacyl-tRNA synthetases and is therefore not functional as a suppressor. Concomitant expression of the E. coli glutaminyl-tRNA synthetase gene results in aminoacylation of the suppressor tRNA and its functioning as a suppressor. These results open up the possibility of attempts at regulated suppression of nonsense codons in mammalian cells by regulating expression of the E. coli glutaminyl-tRNA synthetase gene in an inducible, cell-type specific, or developmentally regulated manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.