ArticleMolecular and cellular biology1996
Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 63 citations in OpenAlex.
- Deletions of singular U1 snRNA gene significantly interfere with transcription and 3'-end mRNA formation.PLoS genetics · 2023Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Introns: the "dark matter" of the eukaryotic genome.Frontiers in genetics · 2023Review
- Regulating Divergent Transcriptomes through mRNA Splicing and Its Modulation Using Various Small Compounds.International journal of molecular sciences · 2020Review
- Modulating splicing with small molecular inhibitors of the spliceosome.Wiley interdisciplinary reviews. RNA · 2017Review
- Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps.RNA biology · 2016Article
- Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeast.PLoS genetics · 2015Article
- Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.RNA (New York, N.Y.) · 2014Article
- RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.The EMBO journal · 2013Article
- Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly.Nucleic acids research · 2013Article
- Conformational heterogeneity of the protein-free human spliceosomal U2-U6 snRNA complex.RNA (New York, N.Y.) · 2013Article
- Rearrangements within human spliceosomes captured after exon ligation.RNA (New York, N.Y.) · 2013Article
- Structure and assembly of the SF3a splicing factor complex of U2 snRNP.The EMBO journal · 2012Article
- Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.PloS one · 2011Article
- Invariant U2 snRNA nucleotides form a stem loop to recognize the intron early in splicing.Molecular cell · 2010Article
- Evidence that U2/U6 helix I promotes both catalytic steps of pre-mRNA splicing and rearranges in between these steps.RNA (New York, N.Y.) · 2009Article
- Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts.RNA (New York, N.Y.) · 2007Article
- Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.Genes & development · 2007Article
- U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing.Genes & development · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
U2 small nuclear RNA (snRNA) contains a sequence (GUAGUA) that pairs with the intron branchpoint during splicing. This sequence is contained within a longer invariant sequence of unknown secondary structure and function that extends between U2 and I and stem IIa. A part of this region has been proposed to pair with U6 in a structure called helix III. We made mutations to test the function of these nucleotides in yeast U2 snRNA. Most single base changes cause no obvious growth defects; however, several single and double mutations are lethal or conditional lethal and cause a block before the first step of splicing. We used U6 compensatory mutations to assess the contribution of helix III and found that if it forms, helix III is dispensable for splicing in Saccharomyces cerevisiae. On the other hand, mutations in known protein components of the splicing apparatus suppress or enhance the phenotypes of mutations within the invariant sequence that connect the branchpoint recognition sequence to stem IIa. Lethal mutations in the region are suppressed by Cus1-54p, a mutant yeast splicing factor homologous to a mammalian SF3b subunit. Synthetic lethal interactions show that this region collaborates with the DEAD-box protein Prp5p and the yeast SF3a subunits Prp9p, Prp11p, and Prp21p. Together, the data show that the highly conserved RNA element downstream of the branchpoint recognition sequence of U2 snRNA in yeast cells functions primarily with the proteins that make up SF3 rather than with U6 snRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.