ArticleThe Journal of clinical investigation1996
Adhesion-activating phorbol ester increases the mobility of leukocyte integrin LFA-1 in cultured lymphocytes.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
82 citing papers in PubMed, 334 citations in OpenAlex.
- CD36 recruits α₅β₁ integrin to promote cytoadherence of P. falciparum-infected erythrocytes.PLoS pathogens · 2013Trial
- Cellular Uptake of Phase-Separating Peptide Coacervates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Connexin43 promotes exocytosis of damaged lysosomes through actin remodelling.The EMBO journal · 2024Article
- SKAP2 acts downstream of CD11b/CD18 and regulates neutrophil effector function.Frontiers in immunology · 2024Article
- The bioenergetics of integrin-based adhesion, from single molecule dynamics to stability of macromolecular complexes.Computational and structural biotechnology journal · 2020Review
- Dynamics of Mechanosensitive Nascent Adhesion Formation.Biophysical journal · 2019Article
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- The effect of healing phenotype-inducing cytokine formulations within soft hydrogels on encapsulated monocytes and incoming immune cells.RSC advances · 2019Article
- T-Cell Mechanobiology: Force Sensation, Potentiation, and Translation.Frontiers in physics · 2019Article
- Dynamics of the actin cytoskeleton mediates receptor cross talk: An emerging concept in tuning receptor signaling.The Journal of cell biology · 2016Review
- Action and Traction: Cytoskeletal Control of Receptor Triggering at the Immunological Synapse.Frontiers in immunology · 2016Review
- Article
- CX3CL1, a chemokine finely tuned to adhesion: critical roles of the stalk glycosylation and the membrane domain.Biology open · 2014Article
- Membrane protein dynamics and functional implications in mammalian cells.Current topics in membranes · 2013Article
- Biophysical description of multiple events contributing blood leukocyte arrest on endothelium.Frontiers in immunology · 2013Article
- Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion.The Journal of cell biology · 2012Article
- Lateral mobility of individual integrin nanoclusters orchestrates the onset for leukocyte adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- CD151 restricts the α6 integrin diffusion mode.Journal of cell science · 2012Article
- Regulation of integrin affinity on cell surfaces.The EMBO journal · 2011Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lymphocytes activate adhesion to intracellular adhesion mlecule 1 (ICAM-1) via leukocyte function associated antigen 1 (LFA-1), their major beta 2 integrin, in response to PMA (phorbol 12-myristate 13-acetate) without an increase in the number of receptors expressed. The molecular details of the mechanism are unknown. To determine the effect of PMA activation on LFA-1 movement within the plasma membrane, we used the single particle tracking technique to measure the diffusion rate of LFA-1 molecules on EBV-transformed B cells before and after PMA activation. Diffusion of LFA-1 on unactivated cells was restricted compared to CR1 (CD35), another transmembrane protein of equivalent size. PMA caused a 10-fold increase in the diffusion rate of LFA-1 without any effect on CD35. The increased LFA-1 motion induced by PMA was random, not directed, indicating that it was due to a release of constraints rather than the application of forces. The diffusion rates of LFA-1 are consistent with cytoskeletal attachment before and free diffusion after PMA. Cytochalasin D led to an equivalent increase in mobility and, at low doses, stimulated adhesion, implying that the nonadhesive state of LFA-1 is actively maintained by the lymphocyte cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.