ArticleThe EMBO journal1996
FhuA, a transporter of the Escherichia coli outer membrane, is converted into a channel upon binding of bacteriophage T5.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 99 citations in OpenAlex.
- Isolation and Characterization of the Polyvalent Enterobacteria-Infecting Phage Cit2 with Potential for Biocontrol Applications.ACS omega · 2026Article
- Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu.Frontiers in microbiology · 2024Article
- Functional characterization of the ABC transporters and transposable elements of an uncultured Paracoccus sp. recovered from a hydrocarbon-polluted soil metagenome.Folia microbiologica · 2023Article
- Structural basis of bacteriophage T5 infection trigger andScience advances · 2023Article
- Structural basis for host recognition and superinfection exclusion by bacteriophage T5.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Development of Bacteriophage Resistance inViruses · 2021Article
- New evolving strategies revealed by transcriptomic analysis of a furMicrobial biotechnology · 2020Article
- Aberrantly Large Single-Channel Conductance of Polyhistidine Arm-Containing Protein Nanopores.Biochemistry · 2017Article
- Sequence Analysis of Hypothetical Proteins fromGenomics & informatics · 2016Article
- Article
- Vibrio vulnificus bacteriophage SSP002 as a possible biocontrol agent.Applied and environmental microbiology · 2014Article
- Structure and functional analysis of the host recognition device of lactococcal phage tuc2009.Journal of virology · 2013Article
- Does the lipid environment impact the open-state conductance of an engineered β-barrel protein nanopore?Biochimica et biophysica acta · 2013Article
- Article
- TonB-dependent transporter FhuA in planar lipid bilayers: partial exit of its plug from the barrel.Biochemistry · 2012Article
- Redesign of a plugged beta-barrel membrane protein.The Journal of biological chemistry · 2011Article
- Visualizing the structural changes of bacteriophage Epsilon15 and its Salmonella host during infection.Journal of molecular biology · 2010Article
- Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- FhuA (TonA), the career of a protein.Journal of bacteriology · 2009Review
- The iron-siderophore transporter FhuA is the receptor for the antimicrobial peptide microcin J25: role of the microcin Val11-Pro16 beta-hairpin region in the recognition mechanism.The Biochemical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Escherichia coli outer membrane protein FhuA catalyzes the transport of Fe3+(-)ferrichrome and is the receptor of phage T5 and phi 80. The purified protein inserted into planar lipid bilayers showed no channel activity. Binding of phage T5 and FhuA resulted in the appearance of high conductance ion channels. The electrophysiological characteristics of the channels (conductance, kinetic behavior, substates, ion selectivity including the effect of ferrichrome) showed similarities with those of the channel formed by a FhuA derivative from which the 'gating loop' (delta 322-355) had been removed. binding of phage T5 to FhuA in E.coli cells conferred SDS sensitivity to the bacteria, suggesting that such channels also exist in vivo. These data suggest that binding of T5 to loop 322-355 of FhuA, which constitutes the T5 binding site, unmasks an inner channel in FhuA. Both T5 and ferrichrome bind to the closed state of the channel but only T5 can trigger its opening.
Indexed as
Identifiers
8617231PMC450102W66814248What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.