ArticleThe EMBO journal1996
The actin binding site of thymosin beta 4 mapped by mutational analysis.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Thymosin β4 promotes zebrafish Mauthner axon regeneration by facilitating actin polymerization through binding to G-actin.BMC biology · 2024Article
- Review
- PIPCommunications biology · 2022Article
- Effects of thymosin β4-derived peptides on migration and invasion of ovarian cancer cells.Genes & genomics · 2021Article
- Cloning, Expression and Effects ofInternational journal of molecular sciences · 2019Article
- The Lack of WIP Binding to Actin Results in Impaired B Cell Migration and Altered Humoral Immune Responses.Cell reports · 2018Article
- Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.Cell · 2017Article
- Binding of WIP to actin is essential for T cell actin cytoskeleton integrity and tissue homing.Molecular and cellular biology · 2014Article
- Binding of the WASP/N-WASP-interacting protein WIP to actin regulates focal adhesion assembly and adhesion.Molecular and cellular biology · 2014Article
- miR-8 controls synapse structure by repression of the actin regulator enabled.Development (Cambridge, England) · 2014Article
- Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation.Nature structural & molecular biology · 2013Article
- Expression patterns of Thymosin β4 and cancer stem cell marker CD133 in ovarian cancers.Pathology oncology research : POR · 2013Article
- The Escherichia coli-derived thymosin β4 concatemer promotes cell proliferation and healing wound in mice.BioMed research international · 2013Article
- Identification and characterization of the actin-binding motif of phostensin.International journal of molecular sciences · 2012Article
- How a single residue in individual β-thymosin/WH2 domains controls their functions in actin assembly.The EMBO journal · 2012Article
- Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton.Molecular biology of the cell · 2012Article
- Direct dynamin-actin interactions regulate the actin cytoskeleton.The EMBO journal · 2010Article
- Article
- Roles of thymosins in cancers and other organ systems.World journal of surgery · 2005Review
- A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We characterized in detail the actin binding site of the small actin-sequestering protein thymosin beta 4 (T beta 4) using chemically synthesized full-length T beta 4 variants. The N-terminal part (residues 1-16) and a hexapeptide motif (residues 17-22) form separate structural entities. In both, we identified charged and hydrophobic residues that participate in the actin interaction using chemical cross-linking, complex formation in native gels and actin-sequestering experiments. Quantitative data on the activity of the variants and circular dichroism experiments allow to present a model in which the N-terminal part needs to adopt an alpha-helix for actin binding and interacts through a patch of hydrophobic residues (6M-I-F12) on one side of this helix. Also, electrostatic contacts between actin and lysine residues 18, in the motif, and 14, in the N-terminal alpha-helix, appear important for binding. The residues critical for contacting actin are conserved throughout the beta-thymosin family and in addition to this we identify a similar pattern in the C-terminal headpiece of villin and dematin.
Indexed as
Identifiers
8617195PMC449934What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.