Evidence map›Paper›PMID 8616262›Full record

ArticlePlant molecular biology1996

Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds.

C O Lim, S I Lee, W S Chung, S H Park, I Hwang, M J Cho

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

C O LimPlant Molecular Biology and Biotechnology Research Center, Chinju, Korea.
S I Lee
W S Chung
S H Park
I Hwang
M J Cho

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.

Indexed as

Amino Acid SequenceBase SequenceBrassicaCysteine Proteinase InhibitorsDNA, ComplementaryEscherichia coliGene DosageGenes, PlantMolecular Sequence DataMultigene FamilyPapainPlant ProteinsPlant ShootsRecombinant Fusion ProteinsSequence Analysis, DNASequence Homology, Amino AcidBCPI-1 protein, Brassica rapaCysteine Proteinase InhibitorsDNA, ComplementaryPapainPlant ProteinsRecombinant Fusion Proteins

Identifiers

PMID8616262

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.