ArticlePlant molecular biology1996
Transgenic rice established to express corn cystatin exhibits strong inhibitory activity against insect gut proteinases.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 114 citations in OpenAlex.
- Differential expression of genes during recovery of Nicotiana tabacum from tomato leaf curl Gujarat virus infection.Planta · 2023Article
- CRISPR/Cas9 Guided Mutagenesis ofInternational journal of molecular sciences · 2021Article
- The wheat multidomain cystatin TaMDC1 displays antifungal, antibacterial, and insecticidal activities in planta.Plant cell reports · 2018Article
- Phytocystatins: Defense Proteins against Phytophagous Insects and Acari.International journal of molecular sciences · 2016Review
- Characterization of Solanum tuberosum multicystatin and the significance of core domains.The Plant cell · 2013Article
- Molecular analysis of maize cystatin expression as fusion product in Escherichia coli.Physiology and molecular biology of plants : an international journal of functional plant biology · 2012Article
- Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins.The Plant cell · 2009Article
- Engineering sugarcane cultivars with bovine pancreatic trypsin inhibitor (aprotinin) gene for protection against top borer (Scirpophaga excerptalis Walker).Plant cell reports · 2009Article
- Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilus oryzae.Transgenic research · 2003Article
- Transfection of COS cells with human cystatin cDNA and its effect on HSV-1 replication.Annals of the New York Academy of Sciences · 1998Article
- Friends and relations of the cystatin superfamily--new members and their evolution.Protein science : a publication of the Protein Society · 1997Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corn cystatin (CC), a phytocystatin, shows a wide inhibitory spectrum against various cysteine proteinases. We produced transgenic rice plants by introducing CC cDNA under CaMV 35S promoter as a first step to obtain a rice plant with insecticidal activity. This attempt was based on the observation that many insect pests, especially Coleoptera, have cysteine proteinases, probably digestive enzymes, and also that oryzacystatin, an intrinsic rice cystatin, shows a narrow inhibition spectrum and is present in ordinary rice seeds in insufficient amounts to inhibit the cysteine proteinases of rice insect pests. The transgenic rice plants generated contained high levels of CC mRNA and CC protein in both seeds and leaves, the CC protein content of the seed reaching ca. 2% of the total heat soluble protein. We also recovered CC activity from seeds and found that the CC fraction efficiently inhibited both papain and cathepsin H, whereas the corresponding fraction from non-transformed rice seeds showed much lower or undetectable inhibitory activities against these cysteine proteinases. Furthermore, CC prepared from transgenic rice plants showed potent inhibitory activity against proteinases that occur in the gut of the insect pest, Sitophilus zeamais.
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