ArticlePlant molecular biology1996
The conserved ELK-homeodomain of KNOTTED-1 contains two regions that signal nuclear localization.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 55 citations in OpenAlex.
- PagKNAT5a promotes plant growth by enhancing xylem cell elongation and secondary wall formation in poplar.Horticulture research · 2025Article
- Identification and expression analysis of the KNOX genes during organogenesis and stress responseness in Camellia sinensis (L.) O. Kuntze.Molecular genetics and genomics : MGG · 2023Article
- Hormonal Regulatory Patterns of LaKNOXs and LaBEL1 Transcription Factors Reveal Their Potential Role in Stem Bulblet Formation in LA Hybrid Lily.International journal of molecular sciences · 2021Article
- Characterization of VvPAL-like promoter from grapevine using transgenic tobacco plants.Functional & integrative genomics · 2016Article
- GmSBH1, a homeobox transcription factor gene, relates to growth and development and involves in response to high temperature and humidity stress in soybean.Plant cell reports · 2015Article
- Transcriptional, posttranscriptional, and posttranslational regulation of SHOOT MERISTEMLESS gene expression in Arabidopsis determines gene function in the shoot apex.Plant physiology · 2015Article
- Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana.Nucleic acids research · 2006Article
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- KNAT6 gene of Arabidopsis is expressed in roots and is required for correct lateral root formation.Plant molecular biology · 2004Article
- Overexpression of a knotted-like homeobox gene of potato alters vegetative development by decreasing gibberellin accumulation.Plant physiology · 2003Article
- Translational fusions with the engrailed repressor domain efficiently convert plant transcription factors into dominant-negative functions.Nucleic acids research · 2002Article
- Functional analysis of the conserved domains of a rice KNOX homeodomain protein, OSH15.The Plant cell · 2001Article
- The expression of Brostm, a KNOTTED1-like gene, marks the cell type and timing of in vitro shoot induction in Brassica oleracea.Plant molecular biology · 2001Article
- Nuclear localization of NPR1 is required for activation of PR gene expression.The Plant cell · 2000Article
- The Dlx3 protein harbors basic residues required for nuclear localization, transcriptional activity and binding to Msx1.Journal of cell science · 2000Article
- The conserved KNOX domain mediates specificity of tobacco KNOTTED1-type homeodomain proteins.The Plant cell · 1999Article
- Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis.Plant molecular biology · 1999Article
- The dominant developmental mutants of tomato, Mouse-ear and Curl, are associated with distinct modes of abnormal transcriptional regulation of a Knotted gene.The Plant cell · 1997Article
- A gene fusion at a homeobox locus: alterations in leaf shape and implications for morphological evolution.The Plant cell · 1997Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear localization serves as a regulatory mechanism in the activity of several transcription factors. KNOTTED-1 (Kn1) is a homeodomain protein likely to regulate vegetative development in maize. At least twelve genes related to Kn1 are known in maize and six in Arabidopsis. Ectopic expression of the maize, rice and Arabidopsis Kn1-related genes have been shown to alter cell fate determination. In this paper, we study the nuclear localization capabilities of the Kn1 homeodomain and the proximal amino acid residues (the ELK region) which is highly conserved among Kn1-related homeodomain proteins. The ELK homeodomain (ELK-HD) of Kn1 was fused to the reporter gene uidA encoding the bacterial enzyme beta-glucuronidase (GUS) and transformed into tobacco and onion cells. Quantitation of GUS activity in nuclear and total protein extracts from transgenic tobacco revealed a highly localized GUS activity in the nucleus for the ELK-HD/GUS fusion protein, as compared to the basal level of GUS activity in the nucleus for the GUS only protein. The ELK-HD/GUS transformants showed no unusual characteristics, thus indicating that expression of the putative Kn1 DNA-binding domain fused to GUS may be insufficient to create a dominant negative phenotype. Histochemical analysis of the onion epidermal cells transfected by particle bombardment demonstrated that greater than 50 % of the transformed onion epidermal cells showed higher levels of GUS staining in the nucleus relative to the cytoplasm. Deletion analysis of the ELK-HD revealed that the Kn1 homeodomain comprising the three predicted alpha-helices and the conserved ELK domain can each function independently as nuclear localization signals.
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