ArticleThe Biochemical journal1996
Acidic phospholipids inhibit the intramolecular association between the N- and C-terminal regions of vinculin, exposing actin-binding and protein kinase C phosphorylation sites.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 149 citations in OpenAlex.
- Bottom-up reconstitution of focal adhesion complexes.The FEBS journal · 2022Review
- Activated Protein Kinase C (PKC) Is Persistently Trafficked with Epidermal Growth Factor (EGF) Receptor.Biomolecules · 2020Article
- Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.eLife · 2020Article
- α-Catenin homodimers are recruited to phosphoinositide-activated membranes to promote adhesion.The Journal of cell biology · 2017Article
- Vinculin in cell-cell and cell-matrix adhesions.Cellular and molecular life sciences : CMLS · 2017Review
- Mechanosensitive Conformation of Vinculin Regulates Its Binding to MAPK1.Biophysical journal · 2017Article
- A Structural Model for Vinculin Insertion into PIPStructure (London, England : 1993) · 2017Article
- Binding of Vinculin to Lipid Membranes in Its Inhibited and Activated States.Biophysical journal · 2016Article
- Differential lipid binding of vinculin isoforms promotes quasi-equivalent dimerization.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.The Journal of biological chemistry · 2016Review
- Lipid binding promotes oligomerization and focal adhesion activity of vinculin.The Journal of cell biology · 2014Article
- Phosphorylation at Y1065 in vinculin mediates actin bundling, cell spreading, and mechanical responses to force.Biochemistry · 2014Article
- Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes.Journal of cell science · 2014Article
- Vinculin phosphorylation at Tyr1065 regulates vinculin conformation and tension development in airway smooth muscle tissues.The Journal of biological chemistry · 2014Article
- HAb18G/CD147 regulates vinculin-mediated focal adhesion and cytoskeleton organization in cultured human hepatocellular carcinoma cells.PloS one · 2014Article
- Review
- Dimer asymmetry defines α-catenin interactions.Nature structural & molecular biology · 2013Article
- The C-terminal tail domain of metavinculin, vinculin's splice variant, severs actin filaments.The Journal of cell biology · 2012Article
- The vinculin C-terminal hairpin mediates F-actin bundle formation, focal adhesion, and cell mechanical properties.The Journal of biological chemistry · 2011Article
- Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion.BMC cell biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chick vinculin polypeptides expressed in Escherichia coli as glutathione S-transferase (GST) fusion proteins have been used to identify the sites involved in the intramolecular association between the 90 kDa N-terminal head and the 30 kDa C-terminal tail region of the vinculin molecule. Fusion proteins spanning vinculin residues 1-258 and 1-398, immobilized on glutathione-agarose beads, were shown to bind a C-terminal vinculin polypeptide spanning residues 881-1066 (liberated from GST by thrombin cleavage). However, the C-terminal polypeptide did not bind to a fusion protein spanning residues 399-881 or to itself. Binding was dependent on residues 167-207 within the N-terminal polypeptide, a sequence also essential for talin binding. Conversely, the 90 kDa head polypeptide was shown to bind to residues 1029-1036 in the tail region of vinculin. The association of the head and tail was inhibited by acidic, but not neutral, phospholipids. Pre-incubation of vinculin with acidic phospholipids exposed the binding site for F-actin and a phosphorylation site for protein kinase C. The phosphorylation site was located in the tail region of the vinculin molecule. These results raise the possibility that acidic phospholipids play a role in regulating the activity of vinculin and therefore the assembly of both cell-cell and cell-matrix adherens-type junctions.
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