ArticleNucleic acids research1996
In vitro generation of a circular exon from a linear pre-mRNA transcript.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Diagnostic value of circRNAs as effective biomarkers in human cardiovascular disease: an updated meta-analysis.International journal of medical sciences · 2022Pooled it
- Self-splicing RNA circularization facilitated by intact group I and II introns.Nature communications · 2025Article
- Emerging role and clinical applications of circular RNAs in human diseases.Functional & integrative genomics · 2025Review
- Expanding the Potential of Circular RNA (CircRNA) Vaccines: A Promising Therapeutic Approach.International journal of molecular sciences · 2025Review
- A Review on Circular RNA Translation and Its Implications in Disease.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Viroids and Retrozymes: Plant Circular RNAs Capable of Autonomous Replication.Plants (Basel, Switzerland) · 2024Review
- Cellular functions and biomedical applications of circular RNAs.Acta biochimica et biophysica Sinica · 2024Review
- Advanced approaches of the use of circRNAs as a replacement for cancer therapy.Non-coding RNA research · 2024Review
- Micropeptides: origins, identification, and potential role in metabolism-related diseases.Journal of Zhejiang University. Science. B · 2023Review
- Astaxanthin Alleviates Foam Cell Formation and Promotes Cholesterol Efflux in Ox-LDL-Induced RAW264.7 Cells via CircTPP2/miR-3073b-5p/ABCA1 Pathway.Molecules (Basel, Switzerland) · 2023Article
- Article
- Review
- Insights Into the Involvement of Circular RNAs in Autoimmune Diseases.Frontiers in immunology · 2021Review
- Circular RNA: BiosynthesisFrontiers in bioengineering and biotechnology · 2021Review
- Biogenesis and functions of circular RNAs and their role in diseases of the female reproductive system.Reproductive biology and endocrinology : RB&E · 2020Review
- Identification of circular RNAs in the ovarian follicles of Meishan and Duroc sows during the follicular phase.Journal of ovarian research · 2020Article
- The Non-Coding RNA Landscape of Plasma Cell Dyscrasias.Cancers · 2020Review
- Emerging Concepts and Challenges in Rheumatoid Arthritis Gene Therapy.Biomedicines · 2020Review
- Past, present, and future of circRNAs.The EMBO journal · 2019Review
- Research progress on circularRNAs in pancreatic cancer: emerging but promising.Cancer biology & therapy · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent findings have firmly established the existence of circular exons in vivo. We were interested in the possible splicing mechanism by which these unusual mRNA molecules could be created in vitro, though no biological relevance has been attached to their existence as yet. In this report we demonstrate that a modified synthetic linear yeast ACT1 transcript whose sequence begins with the 3'-part of its original intron, is continued by 247 nt of exon sequence and terminates with the 5'-part of its intron will generate a circular exon when introduced to standard in vitro splicing reactions in whole cell splice extracts from Saccharomyces cerevisiae. The formation of a circular exon was found to be independent of specific circular or secondary structures of the pre-mRNA transcript. We hypothesize that circular exons which are found in vivo may be generated from pre-mRNAs which derive from rare events of transcription initiation within an intron.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.