ArticleNucleic acids research1996
The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 93 citations in OpenAlex.
- Article
- Mapping Open Chromatin in Trypanosoma brucei Using ATAC-Seq.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Pedal to the Metal: Nuclear Splicing Bodies Turbo-Charge VSG mRNA Production in African Trypanosomes.Frontiers in cell and developmental biology · 2022Review
- Identification of a novel base J binding protein complex involved in RNA polymerase II transcription termination in trypanosomes.PLoS genetics · 2020Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Genome-wide function of MCM-BP in Trypanosoma brucei DNA replication and transcription.Nucleic acids research · 2019Article
- Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly.Parasites & vectors · 2019Review
- The suppressive cap-binding complex factor 4EIP is required for normal differentiation.Nucleic acids research · 2018Article
- Transcriptional Regulation of Telomeric Expression Sites and Antigenic Variation in Trypanosomes.Current genomics · 2018Review
- The role of genomic location and flanking 3'UTR in the generation of functional levels of variant surface glycoprotein in Trypanosoma brucei.Molecular microbiology · 2017Article
- Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei.PLoS pathogens · 2017Article
- A new strategy of RNA interference that targets heterologous sequences reveals CITFA1 as an essential component of class I transcription factor A in Trypanosoma brucei.Eukaryotic cell · 2014Article
- Article
- Development of an efficient in vitro transcription system for bloodstream form Trypanosoma brucei reveals life cycle-independent functionality of class I transcription factor A.Molecular and biochemical parasitology · 2012Article
- Article
- The role of deadenylation in the degradation of unstable mRNAs in trypanosomes.Nucleic acids research · 2009Article
- Differential expression of a protease gene family in African trypanosomes.Molecular and biochemical parasitology · 2009Article
- Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects.Molecular and biochemical parasitology · 2005Article
- Mitochondrial DNA ligases of Trypanosoma brucei.Eukaryotic cell · 2005Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
African trypanosomes are extracellular protozoan parasites that are transmitted from one mammalian host to the next by tsetse flies. Bloodstream forms express variant surface glycoprotein (VSG); the tsetse fly (procyclic) forms express instead the procyclic acidic repetitive protein (PARP). PARP mRNA is abundant in procyclic forms and almost undetectable in blood-stream forms. Post-transcriptional mechanisms are mainly responsible for PARP mRNA regulation but results of nuclear run-on experiments suggested that transcription might also be regulated. We measured the activity of genomically-integrated PARP, VSG and rRNA promoters in permanently-transformed blood-stream and procyclic form trypanosomes, using reporter gene constructs that showed no post-transcriptional regulation. When the constructs were integrated in the rRNA non-transcribed spacer, the ribosomal RNA and VSG promoters were not developmentally regulated, but integration at the PARP locus reduced rRNA promoter activity in bloodstream forms. PARP promoter activity was 5-fold down-regulated in bloodstream forms when integrated at either site. Regulation was probably at the level of transcriptional initiation, but elongation through plasmid vector sequences was also reduced.
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