ArticleInfection and immunity1996
Developmental changes in the expression of Leishmania chagasi gp63 and heat shock protein in a human macrophage cell line.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- IFN-λ3 is induced byInfection and immunity · 2024Article
- HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics.Memorias do Instituto Oswaldo Cruz · 2016Article
- Receptor-mediated phagocytosis of Leishmania: implications for intracellular survival.Trends in parasitology · 2012Review
- Efficacy of synthetic peptides RP-1 and AA-RP-1 against Leishmania species in vitro and in vivo.Antimicrobial agents and chemotherapy · 2012Article
- The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania.Microbes and infection · 2011Article
- Mammalian antimicrobial peptide influences control of cutaneous Leishmania infection.Cellular microbiology · 2011Article
- Macrophage and T-cell gene expression in a model of early infection with the protozoan Leishmania chagasi.PLoS neglected tropical diseases · 2008Article
- The major surface protease (MSP or GP63) in the intracellular amastigote stage of Leishmania chagasi.Molecular and biochemical parasitology · 2008Article
- Calcium-dependent proteolytic activity of a cysteine protease caldonopain is detected during Leishmania infection.Molecular and cellular biochemistry · 2006Article
- Role of Leishmania (Leishmania) chagasi amastigote cysteine protease in intracellular parasite survival: studies by gene disruption and antisense mRNA inhibition.BMC molecular biology · 2005Article
- Iron superoxide dismutases targeted to the glycosomes of Leishmania chagasi are important for survival.Infection and immunity · 2003Article
- Preclinical assessment of the efficacy of mycograb, a human recombinant antibody against fungal HSP90.Antimicrobial agents and chemotherapy · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability of the protozoan Leishmania chagasi to infect a vertebrate host depends on its ability to survive intracellularly in a mammalian macrophage. Novel patterns of gene expression are probably important for conversion from the extracellular promastigote to the obligate intracellular amastigote parasite form. We found that the human macrophage-like cell line U937 provided an in vitro model of phagocytosis of L. chagasi promastigotes and intracellular conversion to amastigotes, allowing examination of parasite protein and RNA expression. The Leishmania surface protease gp63 assumed three isoforms during stage conversion, and a 64-kDa form of gp63 not present in promastigotes became the most prominent form in amastigotes. gp63 RNAs derived from the three different classes of msp genes (mspS, mspL, and mspC) were also differentially expressed. Infectious promastigotes contained mRNAs from mspS and mspC genes, whereas converting parasites expressed only mspL and mspC mRNAs. Sequence analysis of clones from an amastigote cDNA library confirmed the presence of gp63 mRNAs only from mspL and mspC class genes in tissue-derived amastigotes. Finally, 24 h after phagocytosis, there was a transient increase in the level of hsp70 and hsp90 proteins that subsequently decreased to baseline; this increase was not due to heat shock alone. We conclude that a unique pattern of selected L. chagasi proteins and RNAs is induced following phagocytosis by macrophages.
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