ArticleThe EMBO journal1996
A preference of histone H1 for methylated DNA.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- DDM1-mediated gene body DNA methylation is associated with inducible activation of defense-related genes in Arabidopsis.Genome biology · 2023Article
- Cytoskeleton structure and total methylation of mouse cardiac and lung tissue during space flight.PloS one · 2018Article
- Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X.Molecular and cellular biology · 2016Article
- Article
- Spatial heterogeneity of dynamics of H1 linker histone.European biophysics journal : EBJ · 2014Article
- What controls nucleosome positions?Trends in genetics : TIG · 2009Review
- Distinctive sequence patterns in metazoan and yeast nucleosomes: implications for linker histone binding to AT-rich and methylated DNA.Nucleic acids research · 2009Article
- DNA methylation affects nuclear organization, histone modifications, and linker histone binding but not chromatin compaction.The Journal of cell biology · 2007Article
- Histone H1 Is required for proper regulation of pyruvate decarboxylase gene expression in Neurospora crassa.Eukaryotic cell · 2003Article
- DNA methylation and histone deacetylation in the control of gene expression: basic biochemistry to human development and disease.Gene expression · 2000Review
- Histone H1 is dispensable for methylation-associated gene silencing in Ascobolus immersus and essential for long life span.Molecular and cellular biology · 2000Article
- Distinct methylation of the interferon gamma (IFN-gamma) and interleukin 3 (IL-3) genes in newly activated primary CD8+ T lymphocytes: regional IFN-gamma promoter demethylation and mRNA expression are heritable in CD44(high)CD8+ T cells.The Journal of experimental medicine · 1998Article
- The methylated DNA binding protein-2-H1 (MDBP-2-H1) consists of histone H1 subtypes which are truncated at the C-terminus.Nucleic acids research · 1997Article
- Genomic imprinting: a chromatin connection.American journal of human genetics · 1997Review
- Aberrant silencing of the CpG island-containing human O6-methylguanine DNA methyltransferase gene is associated with the loss of nucleosome-like positioning.Molecular and cellular biology · 1997Article
- Constitutively methylated CpG dinucleotides as mutation hot spots in the retinoblastoma gene (RB1).American journal of human genetics · 1997Article
- Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
We have identified a clear preference of histone H1 for CpG-methylated DNA, irrespective of DNA sequence. The conditions under which this preference is observed allowed cooperative binding of H1; the H1-DNA complexes formed were shown earlier to be 'tramlines' of two DNA duplexes bridged by an array of H1 molecules, and multiples of these. The preference for methylated DNA is clear in sedimentation assays, which also show that the preference is greater with increased methylation level, and in gel retardation assays with an oligonucleotide containing a single methyl-CpG pair; it is shared by the globular domain which also binds cooperatively to DNA. A small intrinsic preference of H1 for methylated DNA is also apparent in Southwestern assays where the immobilized H1 presumably cannot bind cooperatively. Methylated DNA in H1-DNA complexes was partially protected (relative to unmethylated DNA) against digestion by MspI but not by enzymes whose cutting sites were not methylated, consistent with a direct interaction of H1 with methylated nucleotides; this was also true of GH1-DNA complexes. H1 variants (spH1 and H5) from transcriptionally repressed nuclei have a stronger preference than H1 for methylated DNA, suggesting that this may be relevant to the stabilization of chromatin higher order structure and transcriptional repression.
Indexed as
Identifiers
8612595PMC450082What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.