ArticleThe EMBO journal1996
Structure of the C-terminal end of the nascent peptide influences translation termination.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 116 citations in OpenAlex.
- A mini-hairpin shaped nascent peptide blocks translation termination by a distinct mechanism.Nature communications · 2025Article
- Environment modulates protein heterogeneity through transcriptional and translational stop codon readthrough.Nature communications · 2024Article
- tRNA epitranscriptome determines pathogenicity of the opportunistic pathogenProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Impact of C-terminal amino acid composition on protein expression in bacteria.Molecular systems biology · 2020Article
- The carboxy-terminus, a key regulator of protein function.Critical reviews in biochemistry and molecular biology · 2019Review
- Article
- Refining the Ambush Hypothesis: Evidence That GC- and AT-Rich Bacteria Employ Different Frameshift Defence Strategies.Genome biology and evolution · 2018Article
- Functional analysis of a nonstop mutation in MITF gene identified in a patient with Waardenburg syndrome type 2.Journal of human genetics · 2017Article
- Protein charge distribution in proteomes and its impact on translation.PLoS computational biology · 2017Article
- eIF5A Functions Globally in Translation Elongation and Termination.Molecular cell · 2017Article
- A Possible Role of the Full-Length Nascent Protein in Post-Translational Ribosome Recycling.PloS one · 2017Article
- Article
- Secondary Structure across the Bacterial Transcriptome Reveals Versatile Roles in mRNA Regulation and Function.PLoS genetics · 2015Article
- YoeB toxin is activated during thermal stress.MicrobiologyOpen · 2015Article
- Evidence of efficient stop codon readthrough in four mammalian genes.Nucleic acids research · 2014Article
- Interactions between HIV-1 Vif and human ElonginB-ElonginC are important for CBF-β binding to Vif.Retrovirology · 2013Article
- Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- Alternative fates of paused ribosomes during translation termination.The Journal of biological chemistry · 2011Article
- Subcellular protein localization by using a genetically encoded fluorescent amino acid.Chembiochem : a European journal of chemical biology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The efficiency of translation termination at NNN NNN UGA A stop codon contexts has been determined in Escherichia coli. No general effects are found which can be attributed directly to the mRNA sequences itself. Instead, termination is influenced primarily by the amino acids at the C-terminal end of the nascent peptide, which are specified by the two codons at the 5' side of UGA. For the penultimate amino acid (-2 location), charge and hydrophobicity are important. For the last amino acid (-1 location), alpha-helical, beta-strand and reverse turn propensities are determining factors. The van der Waals volume of the last amino acid can affect the relative efficiency of stop codon readthrough by the wild-type and suppressor forms of tRNA(Trp) (CAA). The influence of the -1 and -2 amino acids is cooperative. Accumulation of an mRNA degradation intermediate indicates mRNA protection by pausing ribosomes at contexts which give inefficient UGA termination. Highly expressed E.coli genes with the UGA A termination signal encode C-terminal amino acids which favour efficient termination. This restriction is not found for poorly expressed genes.
Indexed as
Identifiers
8612594PMC450081W169922246What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.