ArticleThe EMBO journal1996
Identification of an extracellular motif involved in the binding of guanine nucleotides by a glutamate receptor.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 63 citations in OpenAlex.
- Lessons from the physiological role of guanosine in neurodegeneration and cancer: Toward a multimodal mechanism of action?Purinergic signalling · 2025Review
- Neuromodulatory Effects of Guanine-Based Purines in Health and Disease.Frontiers in cellular neuroscience · 2018Review
- Guanosine: a Neuromodulator with Therapeutic Potential in Brain Disorders.Aging and disease · 2016Review
- The Guanine-Based Purinergic System: The Tale of An Orphan Neuromodulation.Frontiers in pharmacology · 2016Review
- Effects of 3 weeks GMP oral administration on glutamatergic parameters in mice neocortex.Purinergic signalling · 2012Article
- Factors affecting guanine nucleotide binding to rat AMPA receptors.Brain research · 2007Article
- Intracerebroventricular guanine-based purines protect against seizures induced by quinolinic acid in mice.Neurochemical research · 2005Article
- ATP inhibits NMDA receptors after heterologous expression and in cultured hippocampal neurons and attenuates NMDA-mediated neurotoxicity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Neuroprotective effect of GMP in hippocampal slices submitted to an in vitro model of ischemia.Cellular and molecular neurobiology · 2002Article
- Interaction of adenosine and guanine derivatives in the rat hippocampus: effects on cyclic AMP levels and on the binding of adenosine analogues and GMP.Neurochemical research · 2000Article
- Compounds extracted from Phyllantus and Jatropha elliptica inhibit the binding of [3H]glutamate and [3H]GMP-PNP in rat cerebral cortex membrane.Neurochemical research · 2000Article
- Study of adenosine A2 receptors in membrane preparations from optic tectum of chicks.Neurochemical research · 1999Article
- Probing the ligand binding domain of the GluR2 receptor by proteolysis and deletion mutagenesis defines domain boundaries and yields a crystallizable construct.Protein science : a publication of the Protein Society · 1998Article
- AMPA receptors and bacterial periplasmic amino acid-binding proteins share the ionic mechanism of ligand recognition.The EMBO journal · 1998Article
- Effects of guanine nucleotides on glutamate-induced chemiluminescence in rat hippocampal slices submitted to hypoxia.Neurochemical research · 1998Article
- Guanine nucleotides inhibit cAMP accumulation induced by metabotropic glutamate receptor activation.Neurochemical research · 1998Article
- Effect of guanine nucleotides on [3H]glutamate binding and on adenylate cyclase activity in rat brain membranes.Neurochemical research · 1997Article
- Molecular modeling of the ligand-binding domains of the NR3A and NR3B subunits of the NMDA receptor.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chick cerebellar kainate (KA) binding protein (KBP), a member of the family of ionotropic glutamate receptors, harbours a glycine-rich (GxGxxG) motif known to be involved in the binding of ATP and GTP to kinases and G proteins respectively. Here, we report that guanine, but not adenine, nucleotides interact with KBP by inhibiting [3H]KA binding in a competitive-like manner, displaying IC50 values in the micromolar range. To locate the GTP binding site, KBP was photoaffinity labelled with [alpha-32P]GTP. The reaction was blocked by KA, glutamate, 6-cyano-7-nitroquinoxaline-2,3-dione and antibodies raised against a peptide containing the glycine-rich motif. Site-directed mutagenesis of residues K72 and Y73 within the glycine-rich motif followed by the expression of the KBP mutants at the surface of HEK 293 cells showed a decrease in GTP binding affinity by factors of 10 and 100 respectively. The binding of [3H]KA to the K72A/T KBP mutants was not affected but binding to the Y73I KBP mutant was decreased by a factor of 10. Accordingly, we propose that the glycine-rich motif of KBP forms part of a guanine nucleotide binding site. We further suggest that the glycine-rich motif is the binding site at which guanine nucleotides inhibit the glutamate-mediated responses of various members of the subfamily of glutamate ionotropic receptors.
Indexed as
Identifiers
8612578PMC450064W11163000What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.