ArticleThe EMBO journal1996
Reconstitution of transcytosis in SLO-permeabilized MDCK cells: existence of an NSF-dependent fusion mechanism with the apical surface of MDCK cells.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 80 citations in OpenAlex.
- Article
- Multiple paralogues of α-SNAP in Giardia lamblia exhibit independent subcellular localization and redistribution during encystation and stress.Parasites & vectors · 2018Article
- A resealed-cell system for analyzing pathogenic intracellular events: perturbation of endocytic pathways under diabetic conditions.PloS one · 2012Article
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- Exocyst requirement for endocytic traffic directed toward the apical and basolateral poles of polarized MDCK cells.Molecular biology of the cell · 2007Article
- Botulinum toxins--cause of botulism and systemic diseases?Veterinary research communications · 2005Review
- Intersectin regulates fission and internalization of caveolae in endothelial cells.Molecular biology of the cell · 2003Article
- Modulation of endocytic traffic in polarized Madin-Darby canine kidney cells by the small GTPase RhoA.Molecular biology of the cell · 1999Article
- Transduction of basolateral-to-apical signals across epithelial cells: ligand-stimulated transcytosis of the polymeric immunoglobulin receptor requires two signals.Molecular biology of the cell · 1999Article
- Syntaxin 13 mediates cycling of plasma membrane proteins via tubulovesicular recycling endosomes.The Journal of cell biology · 1998Article
- Regulation of endosome sorting by a specific PP2A isoform.The Journal of cell biology · 1998Article
- Selective perturbation of apical membrane traffic by expression of influenza M2, an acid-activated ion channel, in polarized madin-darby canine kidney cells.Molecular biology of the cell · 1998Article
- Role of tyrosine phosphorylation in ligand-induced regulation of transcytosis of the polymeric Ig receptor.Molecular biology of the cell · 1998Article
- Epithelial transcytosis of immunoglobulins.Journal of mammary gland biology and neoplasia · 1998Review
- The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells.The Journal of cell biology · 1998Article
- Rab17 regulates membrane trafficking through apical recycling endosomes in polarized epithelial cells.The Journal of cell biology · 1998Article
- Differential localization of syntaxin isoforms in polarized Madin-Darby canine kidney cells.Molecular biology of the cell · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recently, it was demonstrated that delivery from the trans-Golgi network (TGN) to the basolateral surface of Madin-Darby canine kidney (MDCK) cells required N-ethylmaleimide-sensitive factor (NSF)-alpha soluble NSF attachment protein (SNAP)-SNAP receptor (SNARE) complexes, while delivery from the TGN to the apical surface was independent of NSF-alpha SNAP-SNARE. To determine if all traffic to the apical surface of this cell line was NSF independent, we reconstituted the transcytosis of pre-internalized IgA to the apical surface and recycling to the basolateral surface. Transcytosis and the recycling of IgA required ATP and cytosol, and both were inhibited by treatment with N-ethylmaleimide. This inhibition was reversed by the addition of recombinant NSF. Botulinum neurotoxin serotype E, which is known to cleave the 25,000 Da synaptosomal associated protein, inhibited both transcytosis and recycling, although incompletely. We conclude that membrane traffic to a target membrane is not determined by utilizing a single molecular mechanism for fusion. Rather, a target membrane, e.g. the apical plasma membrane of MDCK cells, may use multiple molecular mechanisms to fuse with incoming vesicle.
Indexed as
Identifiers
8612570PMC450054W946040443What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.