ArticleProceedings of the National Academy of Sciences of the United States of America1996
Pre-mRNA splicing in plants: characterization of Ser/Arg splicing factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 89 citations in OpenAlex.
- MERISTEM-DEFECTIVE regulates the balance between stemness and differentiation in the root meristem through RNA splicing control.Development (Cambridge, England) · 2023Article
- Identification of Key Genes in 'Luang Pratahn', Thai Salt-Tolerant Rice, Based on Time-Course Data and Weighted Co-expression Networks.Frontiers in plant science · 2021Article
- Light Regulation of Alternative Pre-mRNA Splicing in Plants.Photochemistry and photobiology · 2017Review
- NMD mechanism and the functions of Upf proteins in plant.Plant cell reports · 2016Review
- Pre-mRNA Splicing in Plants: In Vivo Functions of RNA-Binding Proteins Implicated in the Splicing Process.Biomolecules · 2015Review
- Quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf.PloS one · 2014Article
- The crosstalk between plant microRNA biogenesis factors and the spliceosome.Plant signaling & behavior · 2013Review
- A role for SR proteins in plant stress responses.Plant signaling & behavior · 2011Review
- Two alternatively spliced isoforms of the Arabidopsis SR45 protein have distinct roles during normal plant development.Plant physiology · 2009Article
- A computational survey of candidate exonic splicing enhancer motifs in the model plant Arabidopsis thaliana.BMC bioinformatics · 2007Article
- Molecular characterization and phylogeny of U2AF35 homologs in plants.Plant physiology · 2006Article
- Evolutionary conservation and regulation of particular alternative splicing events in plant SR proteins.Nucleic acids research · 2006Article
- Phosphoproteomics reveals extensive in vivo phosphorylation of Arabidopsis proteins involved in RNA metabolism.Nucleic acids research · 2006Article
- A plethora of plant serine/arginine-rich proteins: redundancy or evolution of novel gene functions?Biochemical Society transactions · 2004Article
- Determinants of plant U12-dependent intron splicing efficiency.The Plant cell · 2004Article
- The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing.Genome biology · 2004Article
- Ectopic expression of atRSZ33 reveals its function in splicing and causes pleiotropic changes in development.Molecular biology of the cell · 2003Article
- Alternative splicing modulation by a LAMMER kinase impinges on developmental and transcriptome expression.The Plant cell · 2003Article
- Genome analysis: RNA recognition motif (RRM) and K homology (KH) domain RNA-binding proteins from the flowering plant Arabidopsis thaliana.Nucleic acids research · 2002Article
- Sorting out the complexity of SR protein functions.RNA (New York, N.Y.) · 2000Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fact that animal introns are not spliced out in plants suggests that recognition of pre-mRNA splice sites differs between the two kingdoms. In plants, little is known about proteins required for splicing, as no plant in vitro splicing system is available. Several essential splicing factors from animals, such as SF2/ASF and SC-35, belong to a family of highly conserved proteins consisting of one or two RNA binding domain(s) (RRM) and a C-terminal Ser/Arg-rich (SR or RS) domain. These animal SR proteins are required for splice site recognition and spliceosome assembly. We have screened for similar proteins in plants by using monoclonal antibodies specific for a phosphoserine epitope of the SR proteins (mAb1O4) or for SF2/ASF. These experiments demonstrate that plants do possess SR proteins, including SF2/ASF-like proteins. Similar to the animal SR proteins, this group of proteins can be isolated by two salt precipitations. However, compared to the animal SR proteins, which are highly conserved in size and number, SR proteins from Arabidopsis, carrot, and tobacco exhibit a complex pattern of intra- and interspecific variants. These plant SR proteins are able to complement inactive HeLa cell cytoplasmic S1OO extracts that are deficient in SR proteins, yielding functional splicing extracts. In addition, plant SR proteins were active in a heterologous alternative splicing assay. Thus, these plant SR proteins are authentic plant splicing factors.
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