ArticleProceedings of the National Academy of Sciences of the United States of America1996
Oxygen supply and oxygen-dependent gene expression in differentiating embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 133 citations in OpenAlex.
- Glioblastoma Stem Cells and Tumour Microenvironment: Interactions Across Hypoxia, Vasculature and Immune Modulation.International journal of molecular sciences · 2026Review
- Hypoxia-induced one-carbon metabolic reprogramming in glioma stem-like cells.Life medicine · 2023Article
- Exploiting moderate hypoxia to benefit patients with brain disease: Molecular mechanisms and translational research in progress.Neuroprotection (Chichester, England) · 2023Article
- Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial-Mesenchymal Transition in Endometrial Epithelial Cells.Biomedicines · 2023Article
- Decreased Inhibition of Proliferation and Induction of Apoptosis in Breast Cancer Cell Lines (T47D and MCF7) from Treatment with Conditioned Medium Derived from Hypoxia-Treated Wharton's Jelly MSCs Compared with Normoxia-Treated MSCs.International journal of hematology-oncology and stem cell research · 2021Article
- Identification of differentially expressed genes in mouse embryonic stem cell under hypoxia.Genes & genomics · 2021Article
- Cigarette Smoke Extract Activates Hypoxia-Inducible Factors in a Reactive Oxygen Species-Dependent Manner in Stroma Cells from Human Endometrium.Antioxidants (Basel, Switzerland) · 2021Article
- Using Advanced Cell Culture Techniques to Differentiate Pluripotent Stem Cells and Recreate Tissue Structures Representative of Teratoma Xenografts.Frontiers in cell and developmental biology · 2021Article
- The Importance of Proper Oxygenation in 3D Culture.Frontiers in bioengineering and biotechnology · 2021Review
- Towards Multi-Organoid Systems for Drug Screening Applications.Bioengineering (Basel, Switzerland) · 2018Review
- Site-specific gene expression patterns in oral cancer.Head & face medicine · 2017Article
- A Simple Method for Labeling Human Embryonic Stem Cells Destined to Lose Undifferentiated Potency.Stem cells translational medicine · 2016Article
- Analytic Models of Oxygen and Nutrient Diffusion, Metabolism Dynamics, and Architecture Optimization in Three-Dimensional Tissue Constructs with Applications and Insights in Cerebral Organoids.Tissue engineering. Part C, Methods · 2016Article
- Electrical and mechanical stimulation of cardiac cells and tissue constructs.Advanced drug delivery reviews · 2016Review
- Hypoxia attenuates the proinflammatory response in colon cancer cells by regulating IκB.Oncotarget · 2015Article
- Hypoxia enhances differentiation of mouse embryonic stem cells into definitive endoderm and distal lung cells.Stem cells and development · 2015Article
- Relationship between reactive oxygen species and autophagy in dormant mouse blastocysts during delayed implantation.Clinical and experimental reproductive medicine · 2014Article
- Hypoxia-inducible hydrogels.Nature communications · 2014Article
- Low oxygen tension enhances endothelial fate of human pluripotent stem cells.Arteriosclerosis, thrombosis, and vascular biology · 2014Article
- Influence of in vitro and in vivo oxygen modulation on β cell differentiation from human embryonic stem cells.Stem cells translational medicine · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastocyst-derived pluripotent mouse embryonic stem cells can differentiate in vitro to form so-called embryoid bodies (EBs), which recapitulate several aspects of murine embryogenesis. We used this in vitro model to study oxygen supply and consumption as well as the response to reduced oxygenation during the earliest stages of development. EBs were found to grow equally well when cultured at 20% (normoxia) or 1% (hypoxia) oxygen during the first 5 days of differentiation. Microelectrode measurements of pericellular oxygen tension within 13- to 14-day-old EBs (diameter 510-890 micron) done at 20% oxygen revealed efficient oxygenation of the EBs' core region. Confocal laser scanning microscopy analysis of EBs incubated with fluorescent dyes that specifically stain living cells confirmed that the cells within an EB were viable. To determine the EBs' capability to sense low oxygen tension and to specifically respond to low ambient oxygen by modulating gene expression we quantified aldolase A and vascular endothelial growth factor (VEGF) mRNAs, since expression of these genes is upregulated by hypoxia in a variety of cells. Compared with the normoxic controls, we found increased aldolase A and VEGF mRNA levels after exposing 8- to 9-day-old EBs to 1% oxygen. We propose that EBs represent a powerful tool to study oxygen-regulated gene expression during the early steps of embryogenesis, where the preimplantation conceptus resides in a fluid environment with low oxygen tension until implantation and vascularization allow efficient oxygenation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.