ArticleThe Journal of clinical investigation1996
Transepithelial water permeability in microperfused distal airways. Evidence for channel-mediated water transport.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 95 citations in OpenAlex.
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- Physiology and pathophysiology of human airway mucus.Physiological reviews · 2022Review
- Modulator Combination Improves In Vitro the Microrheological Properties of the Airway Surface Liquid of Cystic Fibrosis Airway Epithelia.International journal of molecular sciences · 2022Article
- Bicarbonate transport of airway surface epithelia in luminally perfused mice bronchioles.The journal of physiological sciences : JPS · 2022Article
- Role of complement and potential of complement inhibitors in myasthenia gravis and neuromyelitis optica spectrum disorders: a brief review.Journal of neurology · 2021Review
- Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH.The Journal of physiology · 2020Article
- Aquaporins in the lung.Pflugers Archiv : European journal of physiology · 2019Review
- Water permeability of the mammalian cochlea: functional features of an aquaporin-facilitated water shunt at the perilymph-endolymph barrier.Pflugers Archiv : European journal of physiology · 2014Article
- Role of aquaporins in lung liquid physiology.Respiratory physiology & neurobiology · 2007Review
- Stimulation of aquaporin-5 and transepithelial water permeability in human airway epithelium by hyperosmotic stress.Pflugers Archiv : European journal of physiology · 2007Article
- Water permeability in human airway epithelium.Pflugers Archiv : European journal of physiology · 2005Article
- Sodium and chloride concentrations, pH, and depth of airway surface liquid in distal airways.The Journal of general physiology · 2003Article
- Novel role for CFTR in fluid absorption from the distal airspaces of the lung.The Journal of general physiology · 2002Article
- In vivo airway surface liquid Cl- analysis with solid-state electrodes.The Journal of general physiology · 2002Article
- Role of aquaporin water channels in airway fluid transport, humidification, and surface liquid hydration.The Journal of general physiology · 2001Article
- Airway surface liquid: end of the controversy?The Journal of general physiology · 2001Review
- The osmolyte xylitol reduces the salt concentration of airway surface liquid and may enhance bacterial killing.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice.The Journal of physiology · 2000Article
- Osmotic water permeabilities of cultured, well-differentiated normal and cystic fibrosis airway epithelia.The Journal of clinical investigation · 2000Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Water movement across the airway epithelium is important for regulation of the volume and composition of airspace fluid. A novel approach is reported here to measure osmotic and diffusional water permeability in intact airways. Small airways (100-200 microns diameter, 1-2 mm length) from guinea pig lung were microdissected and perfused in vitro using concentric glass holding and perfusion pipettes. For measurement of osmotic water permeability (Pf), the airway lumen was perfused wit PBS (300 mOsM) containing a membrane impermeable fluorophore, fluorescein sulfonate (FS), and the airway was bathed in solutions of specified osmolalities. Pf determination was based on the changes in FS fluorescence at the distal end of the airway resulting from transepithelial water transport. Pf was 4-5 x 10(-3) cm/s at 23 degrees C and independent of lumen flow rate (10-100 nl/min) and the magnitude and direction of the osmotic gradient (bath osmolality 50-600 mOsM). Temperature dependence measurements gave an activation energy of 4.4 kcal/mol (15-37 degrees C). Pf was not altered by 0.3 mM HgCl2 or 50 microM forskolin, but was increased to 31 x 10(-3) cm/s by 100 micrograms/ml amphotericin B, indicating that osmosis is not limited by unstirred layers. Diffusional water permeability (Pd) was measured by H2O/D2O (deuterium oxide) exchange using the H2O/D2O-sensitive fluorescent probe aminonapthelane trisulfonic acid in the lumen. Measured Pd was 3-6 x 10(-6) cm/s at 23 degrees C, indicating significant restriction to water diffusion by unstirred layers. Antibody localization of water channels showed strong expression of the mercurial-insensitive water channel (AQP-4) at the basolateral membrane of airway epithelial cells. These results provide functional evidence that water movement across the distal airway epithelium is mediated by water channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.