ArticleThe Journal of cell biology1996
Inhibition of laminin alpha 1-chain expression leads to alteration of basement membrane assembly and cell differentiation.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed.
- Proteomic alterations in patient bone-derived stromal cells and their secretomes in osteogenesis imperfecta.Molecular genetics and metabolism reports · 2026Article
- Detection of Protein Carbonylation in Gingival Biopsies from Periodontitis Patients with or Without Diabetes Mellitus-A Pilot Study.Dentistry journal · 2025Article
- Laminin α5: a key factor in tumor metastasis.Clinical & experimental metastasis · 2025Review
- Protein retention in the endoplasmic reticulum rescues Aβ toxicity in Drosophila.Neurobiology of aging · 2023Article
- Association of LAMA1 Single-Nucleotide Polymorphisms with Risk of Esophageal Squamous Cell Carcinoma among the Eastern Chinese Population.Journal of oncology · 2023Article
- CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.BMC research notes · 2020Article
- Article
- Surface functionalization of polyurethane scaffolds mimicking the myocardial microenvironment to support cardiac primitive cells.PloS one · 2018Article
- Sera of patients with spontaneous tumour regression and elevated anti-CA I autoantibodies change the gene expression of ECM proteins.Journal of cellular and molecular medicine · 2017Article
- Intestinal Stem Cell Niche Insights Gathered from BothStem cells international · 2017Review
- Spatial organization of the tenascin-C microenvironment in experimental and human cancer.Cell adhesion & migration · 2015Article
- Stable, covalent attachment of laminin to microposts improves the contractility of mouse neonatal cardiomyocytes.ACS applied materials & interfaces · 2014Article
- Involvement of MIF in basement membrane damage in chronically UVB-exposed skin in mice.PloS one · 2014Article
- The union of somatic gonad precursors and primordial germ cells during Caenorhabditis elegans embryogenesis.Developmental biology · 2013Article
- The development of the myotendinous junction. A review.Muscles, ligaments and tendons journal · 2012Article
- Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion.Journal of cell science · 2012Article
- Lama1 mutations lead to vitreoretinal blood vessel formation, persistence of fetal vasculature, and epiretinal membrane formation in mice.BMC developmental biology · 2011Article
- Loss of enteroendocrine cells in mice alters lipid absorption and glucose homeostasis and impairs postnatal survival.The Journal of clinical investigation · 2010Article
- Mutations in Lama1 disrupt retinal vascular development and inner limiting membrane formation.The Journal of biological chemistry · 2010Article
- Perspectives on tissue interactions in development and disease.Current molecular medicine · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the constituent alpha 1 chain of laminin-1, a major component of basement membranes, is markedly regulated during development and differentiation. We have designed an antisense RNA strategy to analyze the direct involvement of the alpha 1 chain in laminin assembly, basement membrane formation, and cell differentiation. We report that the absence of alpha 1-chain expression, resulting from the stable transfection of the human colonic cancer Caco2 cells with an eukaryotic expression vector comprising a cDNA fragment of the alpha 1 chain inserted in an antisense orientation, led to (a) an incorrect secretion of the two other constituent chains of laminin-1, the beta 1/gamma 1 chains, (b) the lack of basement membrane assembly when Caco2-deficient cells were cultured on top of fibroblasts, assessed by the absence of collagen IV and nidogen deposition, and (c) changes in the structural polarity of cells accompanied by the inhibition of an apical digestive enzyme, sucrase-isomaltase. The results demonstrate that the alpha 1 chain is required for secretion of laminin-1 and for the assembly of basement membrane network. Furthermore, expression of the laminin alpha 1-chain gene may be a regulatory element in determining cell differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.