ArticleThe Journal of cell biology1996
In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed, 139 citations in OpenAlex.
- Nucleolar Dynamics During Oogenesis.bioRxiv : the preprint server for biology · 2026Article
- CX-5461 causes nucleolar compaction, alteration of peri- and intranucleolar chromatin arrangement, an increase in both heterochromatin and DNA damage response.Scientific reports · 2022Article
- Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Emerging Roles of SRSF3 as a Therapeutic Target for Cancer.Frontiers in oncology · 2020Review
- LYAR potentiates rRNA synthesis by recruiting BRD2/4 and the MYST-type acetyltransferase KAT7 to rDNA.Nucleic acids research · 2019Article
- Genome Organization in and around the Nucleolus.Cells · 2019Review
- Novel sub-cellular localizations and intra-molecular interactions may define new functions of Mixed Lineage Leukemia protein.Cell cycle (Georgetown, Tex.) · 2018Article
- Internal Associations of the Acidic Region of Upstream Binding Factor Control Its Nucleolar Localization.Molecular and cellular biology · 2017Article
- Article
- Nucleolar organizer regions: genomic 'dark matter' requiring illumination.Genes & development · 2016Review
- Nucleolar DNA: the host and the guests.Histochemistry and cell biology · 2016Review
- The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription.Scientific reports · 2015Article
- Conditional inactivation of Upstream Binding Factor reveals its epigenetic functions and the existence of a somatic nucleolar precursor body.PLoS genetics · 2014Article
- Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression.PLoS genetics · 2013Article
- BLM helicase facilitates RNA polymerase I-mediated ribosomal RNA transcription.Human molecular genetics · 2012Article
- Nuclear-localized calcineurin homologous protein CHP1 interacts with upstream binding factor and inhibits ribosomal RNA synthesis.The Journal of biological chemistry · 2010Article
- Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus.Histochemistry and cell biology · 2010Article
- Histone H1 phosphorylation is associated with transcription by RNA polymerases I and II.The Journal of cell biology · 2010Article
- Chromatin association and regulation of rDNA transcription by the Ras-family protein RasL11a.The EMBO journal · 2010Article
- AMP-activated protein kinase adapts rRNA synthesis to cellular energy supply.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we show that the TATA-binding protein (TBP) is localized in the nucleoplasm and in the nucleolus of mammalian cells, consistent with its known involvement in transcription by RNA polymerase I, II, and III. In the nucleolus of actively growing cells, TBP colocalizes with upstream binding factor (UBF) and RNA polymerase I at the sites of rRNA transcription. During mitosis, when rRNA synthesis is down-regulated, TBP colocalizes with TBP-associated factors for RNA polymerase I (TAF(I)s), UBF, and RNA polymerase I on the chromosomal regions containing the rRNA genes. Treatment of cells with a low concentration of actinomycin D inhibits rRNA synthesis and causes a redistribution of the rRNA genes that become concentrated in clusters at the periphery of the nucleolus. A similar redistribution was observed for the major components of the rRNA transcription machinery (i.e., TBP, TAF(I)s, UBF, and RNA polymerase I), which still colocalized with each other. Furthermore, anti-TBP antibodies are shown to coimmunoprecipitate TBP and TAF(I)63 in extracts prepared from untreated and actinomycin D-treated cells. Collectively, the data indicate that in vivo TBP/promoter selectivity factor, UBF, and RNA polymerase I remain associated with both active and inactive rRNA genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.