ArticleClinical and experimental immunology1996
Selected Th1 and Th2 cytokine mRNA expression by CD4(+) T cells isolated from inflamed human gingival tissues.
Article in Clinical and experimental immunology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 113 citations in OpenAlex.
- Osteocyte RANKL Drives Bone Resorption in Mouse Ligature-Induced Periodontitis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Role of Interleukin-17A in the Pathomechanisms of Periodontitis and Related Systemic Chronic Inflammatory Diseases.Frontiers in immunology · 2022Review
- Single-Cell Analysis of the Periodontal Immune Niche in Type 2 Diabetes.Journal of dental research · 2020Article
- Placental colonization with periodontal pathogens: the potential missing link.American journal of obstetrics and gynecology · 2019Review
- HIV Infection and Compromised Mucosal Immunity: Oral Manifestations and Systemic Inflammation.Frontiers in immunology · 2017Review
- Association between Polymorphisms in Interleukins 4 and 13 Genes and Chronic Periodontitis in a Han Chinese Population.BioMed research international · 2016Article
- Expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis.Journal of dentistry (Tehran, Iran) · 2013Article
- Induction of IL-10-producing CD4+ T-cells in chronic periodontitis.Journal of dental research · 2011Article
- Interferon-gamma promoter hypomethylation and increased expression in chronic periodontitis.Journal of clinical periodontology · 2010Article
- A new inflammatory cytokine on the block: re-thinking periodontal disease and the Th1/Th2 paradigm in the context of Th17 cells and IL-17.Journal of dental research · 2008Review
- Expression of fractalkine (CX3CL1) and its receptor, CX3CR1, in periodontal diseased tissue.Clinical and experimental immunology · 2005Article
- Cytokine profiles of patients infected with Mycobacterium ulcerans and unaffected household contacts.Infection and immunity · 2002Article
- Mixed periodontal Th1-Th2 cytokine profile in Actinobacillus actinomycetemcomitans-specific osteoprotegerin ligand (or RANK-L)- mediated alveolar bone destruction in vivo.Infection and immunity · 2002Article
- Accumulation of human heat shock protein 60-reactive T cells in the gingival tissues of periodontitis patients.Infection and immunity · 2002Article
- Human gingival CD14(+) fibroblasts primed with gamma interferon increase production of interleukin-8 in response to lipopolysaccharide through up-regulation of membrane CD14 and MyD88 mRNA expression.Infection and immunity · 2002Article
- Anti-inflammatory cytokine IL-10 and T cell cytokine profile in periodontitis granulation tissue.Clinical and experimental immunology · 2001Article
- Article
- Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings.Mediators of inflammation · 2000Article
- Cytokine mRNA expression in lesions in cats with chronic gingivostomatitis.Clinical and diagnostic laboratory immunology · 1999Article
- CD4(+) T cells and the proinflammatory cytokines gamma interferon and interleukin-6 contribute to alveolar bone loss in mice.Infection and immunity · 1999Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Elevated numbers of plasma cells are associated with localized and chronically inflamed gingiva of patients with adult periodontitis. However, only limited information is currently available as to how cytokines produced by CD4(+) T cells are involved in these increased B cell responses in affected gingival tissues. When gingival mononuclear cells (GMC) were isolated from inflamed tissues and examined by flow cytometry, approximately 20-30% of lymphocytes were CD4(+) T cells. For the analysis of Th1 and Th2 cytokine expression by these CD4(+) T cells, RNA was extracted and reverse transcriptase-polymerase chain reaction (RT-PCR) was performed by using specific 5' and 3' primers for interferon-gamma (IFN-gamma) and IL-2 (Th1), IL-4, IL-5, IL-6, IL-10 and IL-13 (Th2) and beta-actin (internal control). Two distinct cytokine profiles were noted based on the expression of selected Th1 and Th2 cytokines, where one pattern was represented by expression of mRNA for IFN-gamma, IL-6, IL-10 and IL-13, while the second consisted of mRNA for IFN-gamma, IL-6 and IL-13. In most samples, mRNA for IL-2, IL-4 and IL-5 were not detected by cytokine-specific RT-PCR. When RNA was isolated from CD4(+) T cells of concanavalin A-stimulated peripheral blood mononuclear cells (PBMC) of the same patients and examined by RT-PCR, mRNA for all Th1 and Th2 cytokines were detected. These findings suggest that although human CD4(+) T cells are capable of producing an array of Th1- and Th2-type cytokines, the CD4(+) T cells associated with periodontitis are limited to production of IFN-gamma, IL-6, IL-13 and is some instances IL-10. CD4(+) T cells from diseased periodontal tissues are divisible into two groups based upon whether or not IL-10 is produced, together with IFN-gamma, IL-6 and IL-13.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.