ArticleClinical and experimental immunology1996
Detection of hepatitis C virus (HCV) proteins by immunofluorescence and HCV RNA genomic sequences by non-isotopic in situ hybridization in bone marrow and peripheral blood mononuclear cells of chronically HCV-infected patients.
Article in Clinical and experimental immunology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 106 citations in OpenAlex.
- Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis.Journal of medical microbiology · 2016Article
- Role of macrophages and monocytes in hepatitis C virus infections.World journal of gastroenterology · 2014Review
- HCV enters the twenty-first century.Current infectious disease reports · 2013Article
- Biological significance of HCV in various kinds of lymphoid cells.International journal of microbiology · 2012Article
- Pathogenetic mechanisms of hepatitis C virus-induced B-cell lymphomagenesis.Clinical & developmental immunology · 2012Review
- Human cell types important for hepatitis C virus replication in vivo and in vitro: old assertions and current evidence.Virology journal · 2011Review
- HCV infection and B-cell lymphomagenesis.Advances in hematology · 2011Article
- Hepatitis C virus neuroinvasion: identification of infected cells.Journal of virology · 2009Article
- Cognitive dysfunction in chronic hepatitis C: a review.Digestive diseases and sciences · 2008Review
- Hepatitis C virus productive infection in mononuclear cells from patients with cryoglobulinaemia.Clinical and experimental immunology · 2007Article
- Negative-strand hepatitis C virus (HCV) RNA in peripheral blood mononuclear cells from anti-HCV-positive/HIV-infected women.The Journal of infectious diseases · 2007Article
- Soluble egg antigen of Schistosoma Haematobium induces HCV replication in PBMC from patients with chronic HCV infection.BMC infectious diseases · 2006Article
- Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: binding and entry.World journal of gastroenterology · 2005Article
- Reduced type 1 and type 2 cytokines in antiviral memory T helper function among women coinfected with HIV and HCV.Journal of clinical immunology · 2005Article
- Histological characterization of HCV-associated glomerulopathy in Egyptian patients.International urology and nephrology · 2005Article
- HCV replication in PBMC and its influence on interferon therapy.World journal of gastroenterology · 2003Article
- Detection and analysis of hepatitis C virus sequences in cerebrospinal fluid.Journal of virology · 2002Article
- HCV-RNA positivity in peripheral blood mononuclear cells of patients with chronic HCV infection: does it really mean viral replication?World journal of gastroenterology · 2001Article
- Use of whole blood specimens for routine clinical quantitation of hepatitis C virus RNA does not increase assay sensitivity.Journal of clinical microbiology · 2000Article
- Histological damage in chronic hepatitis C is not related to the extent of infection in the liver.The American journal of pathology · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunofluorescence (IF) to detect HCV antigens and non-isotopic in situ hybridization (NISH) to detect HCV RNA genome were carried out on bone marrow (BM) and peripheral blood (PB) mononuclear cells (MC) of 11 chronically HCV-infected patients. In four patients (36.4%) HCV antigens were detected in monocytes/macrophages as well as in B lymphocytes in both BMMC and PBMC. Positive T lymphocytes in BMMC were found in three of them, but only one patient showed positive T cells in PBMC. NISH invariably demonstrated minus and plus HCV RNA genomic strands either in monocytes/macrophages or B and T lymphocytes in BMMC and PBMC in the four HCV antigen-positive patients and in two further patients not expressing viral proteins in blood MC. IF signals appeared diffusely distributed within the cytoplasm, or as brilliant granules in distinct submembrane areas or else in cytoplasm membrane. Nuclei never stained. Similarly, NISH displayed HCV RNA accumulation restricted to MC cytoplasm only, nuclei being persistently negative. NISH, however, was unable to detect cell membrane signal. Infection of blood MC is a common event in naturally acquired HCV infection, since none of these patients was conditioned by immunomodulating or immunosuppressive therapies. No difference was found in terms of age, length of disease, anti-HCV immune response, type and severity of chronic liver damage between patients with HCV-infected MC and patients without cell infection. These results demonstrate that HCV can infect BMMC and PBMC that represent important extrahepatic sites of virus replication, and may help to explain the immunological abnormalities observed in chronic HCV carriers.
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