ArticleJournal of bacteriology1996
The CCAAT box-binding factor stimulates ammonium assimilation in Saccharomyces cerevisiae, defining a new cross-pathway regulation between nitrogen and carbon metabolisms.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Disordered sequences of transcription factors regulate genomic binding by integrating diverse sequence grammars and interaction types.Nucleic acids research · 2024Article
- "Labile" heme critically regulates mitochondrial biogenesis through the transcriptional co-activator Hap4p inThe Journal of biological chemistry · 2020Article
- Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum.Scientific reports · 2020Article
- The CCAAT-Binding Complex Controls Respiratory Gene Expression and Iron Homeostasis in Candida Glabrata.Scientific reports · 2017Article
- The Iron-Dependent Regulation of the Candida albicans Oxidative Stress Response by the CCAAT-Binding Factor.PloS one · 2017Article
- A wheat CCAAT box-binding transcription factor increases the grain yield of wheat with less fertilizer input.Plant physiology · 2015Article
- Article
- PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii.Journal of experimental botany · 2011Article
- How yeast re-programmes its transcriptional profile in response to different nutrient impulses.BMC systems biology · 2011Article
- A stress response related to the carbon source and the absence of KlHAP2 in Kluyveromyces lactis.Journal of industrial microbiology & biotechnology · 2011Article
- Article
- Proteome analysis of yeast response to various nutrient limitations.Molecular systems biology · 2006Article
- Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
- Novel regulatory function for the CCAAT-binding factor in Candida albicans.Eukaryotic cell · 2005Article
- Steady-state analysis of glucose repression reveals hierarchical expression of proteins under Mig1p control in Saccharomyces cerevisiae.The Biochemical journal · 2005Article
- Expression of the CCAAT-binding factor NF-Y in Caenorhabditis elegans.Journal of molecular histology · 2005Article
- TamA interacts with LeuB, the homologue of Saccharomyces cerevisiae Leu3p, to regulate gdhA expression in Aspergillus nidulans.Molecular genetics and genomics : MGG · 2004Article
- Phosphatidylinositol 4-kinasebeta is critical for functional association of rab11 with the Golgi complex.Molecular biology of the cell · 2004Article
- AnCF, the CCAAT binding complex of Aspergillus nidulans, contains products of the hapB, hapC, and hapE genes and is required for activation by the pathway-specific regulatory gene amdR.Molecular and cellular biology · 1999Article
- Yeast carbon catabolite repression.Microbiology and molecular biology reviews : MMBR · 1998Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Saccharomyces cerevisiae, carbon and nitrogen metabolisms are connected via the incorporation of ammonia into glutamate; this reaction is catalyzed by the NADP-dependent glutamate dehydrogenase (NADP-GDH) encoded by the GDH1 gene. In this report, we show that the GDH1 gene requires the CCAAT box-binding activator (HAP complex) for optimal expression. This conclusion is based on several lines of evidence: (1) overexpression of GDH1 can correct the growth defect of hap2 and hap3 mutants on ammonium sulfate as a nitrogen source, (ii) Northern (RNA) blot analysis shows that the steady-state level of GDH1 mRNA is strongly lowered in a hap2 mutant, (iii) expression of a GDH1-lacZ fusion is drastically reduced in hap mutants, (iv) NADP-GDH activity is several times lower in the hap mutants compared with that in the isogenic wild-type strain, and finally, (v) site-directed mutagenesis of two consensual HAP binding sites in the GDH1 promoter strongly reduces expression of GDH1 and makes it HAP independent. Expression of GDH1 is also regulated by the carbon source, i.e., expression is higher on lactate than on ethanol, glycerol, or galactose, with the lowest expression being found on glucose. Finally, we show that a hap2 mutation does not affect expression of other genes involved in nitrogen metabolism (GDH2, GLN1, and GLN3 encoding, respectively, the NAD-GDH, glutamine synthetase, and a general activator of several nitrogen catabolic genes). The HAP complex is known to regulate expression of several genes involved in carbon metabolism; its role in the control of GDH1 gene expression, therefore, provides evidence for a cross-pathway regulation between carbon and nitrogen metabolisms.
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