ArticleJournal of bacteriology1996
In vitro binding of the Salmonella dublin virulence plasmid regulatory protein SpvR to the promoter regions of spvA and spvR.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Public health data collected by PulseNet support a role forMicrobiology spectrum · 2026Article
- Bimodal Expression of theGenetics · 2018Article
- Association of Three Plasmid-Encoded spv Genes Among Different Salmonella Serotypes Isolated from Different Origins.Indian journal of microbiology · 2013Article
- Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice.PloS one · 2013Article
- The Role of the spv Genes in Salmonella Pathogenesis.Frontiers in microbiology · 2011Article
- Phage WO of Wolbachia: lambda of the endosymbiont world.Trends in microbiology · 2010Review
- SpvC is a Salmonella effector with phosphothreonine lyase activity on host mitogen-activated protein kinases.Molecular microbiology · 2008Article
- The LysR-type transcriptional regulator VirR is required for expression of the virulence gene vapA of Rhodococcus equi ATCC 33701.Journal of bacteriology · 2004Article
- Characterization of the spv locus in Salmonella enterica serovar Arizona.Infection and immunity · 2002Article
- Regulated antigen expression in live recombinant Salmonella enterica serovar Typhimurium strongly affects colonization capabilities and specific CD4(+)-T-cell responses.Infection and immunity · 2001Article
- Renaturation of Bacillus thermoglucosidasius HrcA repressor by DNA and thermostability of the HrcA-DNA complex in vitro.Journal of bacteriology · 2001Article
- Transcriptional activation of the chlorocatechol degradative genes of Ralstonia eutropha NH9.Journal of bacteriology · 1999Article
- Characterization of CorR, a transcriptional activator which is required for biosynthesis of the phytotoxin coronatine.Journal of bacteriology · 1998Article
- Mutational characterization of promoter regions recognized by the Salmonella dublin virulence plasmid regulatory protein SpvR.Journal of bacteriology · 1997Article
- Expression profile and subcellular location of the plasmid-encoded virulence (Spv) proteins in wild-type Salmonella dublin.Infection and immunity · 1997Article
- The spv genes on the Salmonella dublin virulence plasmid are required for severe enteritis and systemic infection in the natural host.Infection and immunity · 1997Article
- Regulation of bacterial virulence gene expression by the host environment.The Journal of clinical investigation · 1997Review
- Plasmid virulence gene expression induced by short-chain fatty acids in Salmonella dublin: identification of rpoS-dependent and rpo-S-independent mechanisms.Journal of bacteriology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The spv regulon of Salmonella dublin is essential for virulence in mice. SpvR, a LysR-type regulator, induces the expression of the spvABCD operon and its own expression in the stationary phase of bacterial growth and in macrophages. We constructed fusion proteins to the maltose-binding protein (MBP) and a His tag peptide (His) to overcome the insolubility and to facilitate purification of SpvR. We demonstrated that both fusion proteins, MBP-SpvR and His-SpvR, were able to induce spvA expression in vivo. MBP-SpvR was produced as soluble protein, whereas His-SpvR was only marginally present in the soluble cell fraction. Affinity chromatography resulted in at least 95% pure MBP-SpvR protein and in an enrichment of His-SpvR. Gel mobility shift assay revealed that the SpvR fusion proteins were able to bind to 125-and 147-bp DNA fragments of the spvA and spvR promoter regions, respectively. DNase I footprint experiments showed that the fusion proteins protected DNA regions of 54 and 50 bp within the spvA and spvR promoter regions, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.