ArticleInfection and immunity1996
Effect of cell polarization and differentiation on entry of Listeria monocytogenes into the enterocyte-like Caco-2 cell line.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 110 citations in OpenAlex.
- Engagement of αFrontiers in microbiology · 2024Article
- Egress of Listeria monocytogenes from Mesenteric Lymph Nodes Depends on Intracellular Replication and Cell-to-Cell Spread.Infection and immunity · 2023Article
- Article
- Differential Interaction between Invasive Thai Group BMicroorganisms · 2022Article
- Invasion and trafficking of hypervirulent group B streptococci in polarized enterocytes.PloS one · 2021Article
- Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps.PLoS pathogens · 2020Article
- Bioengineering NovelFrontiers in bioengineering and biotechnology · 2020Article
- Lactobacillus casei expressing Internalins A and B reduces Listeria monocytogenes interaction with Caco-2 cells in vitro.Microbial biotechnology · 2019Article
- Adhesion to the host cell surface is sufficient to mediateMolecular biology of the cell · 2017Article
- Bacteriocin-ProducingFrontiers in microbiology · 2017Article
- Listeria monocytogenes Inhibits Serotonin Transporter in Human Intestinal Caco-2 Cells.Microbial ecology · 2016Article
- Hypoxia Decreases Invasin-Mediated Yersinia enterocolitica Internalization into Caco-2 Cells.PloS one · 2016Article
- 3-D intestinal scaffolds for evaluating the therapeutic potential of probiotics.Molecular pharmaceutics · 2014Article
- Sensing of barrier tissue disruption with an organic electrochemical transistor.Journal of visualized experiments : JoVE · 2014Article
- Pathogenesis of human enterovirulent bacteria: lessons from cultured, fully differentiated human colon cancer cell lines.Microbiology and molecular biology reviews : MMBR · 2013Review
- Listeriolysin O as a strong immunogenic molecule for the development of new anti-tumor vaccines.Human vaccines & immunotherapeutics · 2013Review
- Article
- Examination of food chain-derived Listeria monocytogenes strains of different serotypes reveals considerable diversity in inlA genotypes, mutability, and adaptation to cold temperatures.Applied and environmental microbiology · 2013Article
- Differential effects of nutritional and non-nutritional therapies on intestinal barrier function in an in vitro model.Journal of gastroenterology · 2012Article
- Systemic response to Campylobacter jejuni infection by profiling gene transcription in the spleens of two genetic lines of chickens.Immunogenetics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The entry of Listeria monocytogenes into the enterocyte-like Caco-2 cell line was studied as a function of cell polarization and differentiation. L. monocytogenes entered through the entire surface of nonpolarized cells and, predominantly, through the basolateral surface of polarized cells based on the following observations: (i) sites of L. monocytogenes invasion paralleled the distribution of the transferrin receptor, a well-known basolateral marker of polarization; (ii) numbers of internalized bacteria decreased dramatically when Caco-2 monolayers cultured beyond confluency were used (about 0.1% of the inoculated bacteria versus 1 to 2% with nonconfluent monolayers); and (iii) L. monocytogenes entry into postconfluent monolayers was greatly enhanced by treating cells with Ca(2)+ -free medium, a procedure that disrupts intercellular junctions and thus exposes the basolateral surface to bacteria. Ethylene glycol-bis (beta-aminoethyl ether)-N, N, N',N' -tetraacetic acid (EGTA) had contradictory effects on L. monocytogenes entry as this reagent opened intercellular junctions but inhibited binding and internalization of bacteria. Finally, the role of the inlAB locus in L. monocytogenes entry was confirmed because and inlAB mutant was 50- to 100-fold less invasive than the parental strain regardless of the monolayer's age. However, the inlAB mutant was still able to enter cells and to induce intracellular actin polymerization. Entry of inlAB bacteria into Caco-2 cells was not inhibited by EGTA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.