ArticleInfection and immunity1996
Cell growth rate regulates expression of group B Streptococcus type III capsular polysaccharide.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 66 citations in OpenAlex.
- Role of pilus proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells.The Journal of biological chemistry · 2013Trial
- Identification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation.Veterinary research · 2025Article
- Surface Structures of Group BMicrobiology spectrum · 2019Review
- A novel suicide shuttle plasmid for Streptococcus suis serotype 2 and Streptococcus equi ssp. zooepidemicus gene mutation.Scientific reports · 2016Article
- Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response.PloS one · 2015Article
- Identification and molecular characterization of a S. agalactiae strain lacking the capsular locus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Membrane topology and DNA-binding ability of the Streptococcal CpsA protein.Journal of bacteriology · 2011Article
- Role of p38 and early growth response factor 1 in the macrophage response to group B streptococcus.Infection and immunity · 2009Article
- Understanding the regulation of Group B Streptococcal virulence factors.Future microbiology · 2009Review
- Bacterial phenotype variants in group B streptococcal toxic shock syndrome.Emerging infectious diseases · 2009Article
- Recombinant group B streptococcus Beta C protein and a variant with the deletion of its immunoglobulin A-binding site are protective mouse maternal vaccines and effective carriers in conjugate vaccines.Infection and immunity · 2007Article
- Transcriptional and proteomic profiles of group B Streptococcus type V reveal potential adherence proteins associated with high-level invasion.Infection and immunity · 2007Article
- The streptococcal Blr and Slr proteins define a family of surface proteins with leucine-rich repeats: camouflaging by other surface structures.Journal of bacteriology · 2006Article
- Sialylation of group B streptococcal capsular polysaccharide is mediated by cpsK and is required for optimal capsule polymerization and expression.Journal of bacteriology · 2005Article
- Characterization of a novel leucine-rich repeat protein antigen from group B streptococci that elicits protective immunity.Infection and immunity · 2005Article
- MtaR, a regulator of methionine transport, is critical for survival of group B streptococcus in vivo.Journal of bacteriology · 2003Article
- Molecular analysis of the Enterococcus faecalis serotype 2 polysaccharide determinant.Journal of bacteriology · 2003Article
- Recognition of pneumolysin by Toll-like receptor 4 confers resistance to pneumococcal infection.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Use of a dynamic in vitro attachment and invasion system (DIVAS) to determine influence of growth rate on invasion of respiratory epithelial cells by group B Streptococcus.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- The serotype of type Ia and III group B streptococci is determined by the polymerase gene within the polycistronic capsule operon.Journal of bacteriology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The capsular polysaccharide (CPS) of group B streptococci (GBS) is an important virulence factor that also serves to protect cells from nonspecific host defense mechanisms. Expression of CPS by GBS, as with other encapsulated bacterial pathogens, is not constitutive but varies during growth in vitro and in primary cultures isolated from different sites of infection. Despite this understanding, little is known about regulation of this surface-expressed carbohydrate antigen in GBS. Here we report that expression of type III CPS by GBS strain M781 grown in continuous culture with a modified chemically defined medium is regulated by growth rate. Cells in steady state at mass doubling times (tds) of 0.8, 1.4, and 1.6 h expressed an average of sixfold more cell-associated CPS than did cells held at tds of 2.3 and 11 h. Strain M781 grown at a td of 1.4 h repeatedly produced more type III CPS than those held at a td of 11.0 h, even when limited for glucose, pyridoxamine, or thiamine. In our studies, > or = 93% of the total CPS expressed by strain M781 was cell associated. Strain M781 grown at a td of 11.0 h (i.e., lowered CPS expression) was susceptible to in vitro complement-mediated opsonophagocytosis and killing by human peripheral blood leukocytes, whereas cells grown at a td of 1.4 h (i.e., higher CPS expression) were not killed unless type III CPS-specific antibody was present. Factors that allow GBS to asymptomatically colonize women yet cause invasive infection to both mother and infant are poorly understood. Our results shed new light on parameters that regulate the pathogenic potential of GBS and may also serve as a way to discern more fully the genetics and biochemistry of GBS capsule synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.