ArticleThe EMBO journal1996
Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 170 citations in OpenAlex.
- A glycolytic intermediate gatekeeps global mRNA decay in Escherichia coli.Nature communications · 2026Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- The world of ribonucleases from pseudomonads: a short trip through the main features and singularities.Microbial biotechnology · 2021Review
- Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Organellar and Secretory Ribonucleases: Major Players in Plant RNA Homeostasis.Plant physiology · 2020Article
- Major contribution of transcription initiation to 5'-end formation of mitochondrial steady-state transcripts in maize.RNA biology · 2019Article
- Post-translational Modifications in Regulation of Chloroplast Function: Recent Advances.Frontiers in plant science · 2017Review
- RBF1, a plant homolog of the bacterial ribosome-binding factor RbfA, acts in processing of the chloroplast 16S ribosomal RNA.Plant physiology · 2014Article
- Arabidopsis chloroplast RNA binding proteins CP31A and CP29A associate with large transcript pools and confer cold stress tolerance by influencing multiple chloroplast RNA processing steps.The Plant cell · 2012Article
- The effect of different 3' untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco.Plant molecular biology · 2011Article
- Selection of Shine-Dalgarno sequences in plastids.Nucleic acids research · 2011Article
- Overaccumulation of the chloroplast antisense RNA AS5 is correlated with decreased abundance of 5S rRNA in vivo and inefficient 5S rRNA maturation in vitro.RNA (New York, N.Y.) · 2011Article
- Long transcripts from dinoflagellate chloroplast minicircles suggest "rolling circle" transcription.The Journal of biological chemistry · 2010Article
- Abnormal physiological and molecular mutant phenotypes link chloroplast polynucleotide phosphorylase to the phosphorus deprivation response in Arabidopsis.Plant physiology · 2009Article
- Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Chloroplast genome sequencing analysis of Heterosigma akashiwo CCMP452 (West Atlantic) and NIES293 (West Pacific) strains.BMC genomics · 2008Article
- A 125 kDa RNase E/G-like protein is present in plastids and is essential for chloroplast development and autotrophic growth in Arabidopsis.Journal of experimental botany · 2008Article
- Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons.The Plant journal : for cell and molecular biology · 2007Article
- The nucleus-encoded factor MCD4 participates in degradation of nonfunctional 3' UTR sequences generated by cleavage of pre-mRNA in Chlamydomonas chloroplasts.Molecular genetics and genomics : MGG · 2007Article
- Integration of chloroplast nucleic acid metabolism into the phosphate deprivation response in Chlamydomonas reinhardtii.The Plant cell · 2007Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the absence of efficient transcription termination correct 3'-end processing is an essential step in the synthesis of stable chloroplast mRNAs in higher plants. We show here that 3'-end processing in vitro involves endonucleolytic cleavage downstream from the mature terminus, followed by exonucleolytic processing to a stem-loop within the 3'-untranslated region. These processing steps require a high molecular weight complex that contains both endoribonucleases and an exoribonuclease. In the presence of ancillary RNA binding proteins the complex correctly processes the 3'-end of precursor RNA. In the absence of these ancillary proteins 3'-end maturation is prevented and plastid mRNAs are degraded. Based on these results we propose a novel mechanism for the regulation of mRNA 3'-end processing and stability in chloroplasts.
Indexed as
Identifiers
8605883PMC450011W289916777What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.