ArticlePlant molecular biology1996
Over-production of the D1:2 protein makes Synechococcus cells more tolerant to photoinhibition of photosystem II.
Article in Plant molecular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Omics-Inferred Partitioning and Expression of Diverse Biogeochemical Functions in a Low-OmSystems · 2021Article
- A Specific Single Nucleotide Polymorphism in the ATP Synthase Gene Significantly Improves Environmental Stress Tolerance of Synechococcus elongatus PCC 7942.Applied and environmental microbiology · 2018Article
- Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco.Frontiers in plant science · 2015Article
- Cyanobacterial psbA gene family: optimization of oxygenic photosynthesis.Cellular and molecular life sciences : CMLS · 2009Review
- The photosynthetic apparatus of Prochlorococcus: Insights through comparative genomics.Photosynthesis research · 2001Article
- Prochlorococcus, a marine photosynthetic prokaryote of global significance.Microbiology and molecular biology reviews : MMBR · 1999Review
- Expression of psbA genes produces prominent 5' psbA mRNA fragments in Synechococcus sp. PCC 7942.Plant molecular biology · 1998Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over-expression of the psbAIII gene encoding for the D1 protein (form II; D1:2) of the photosystem II reaction centre in the Synechococcus sp. PCC 7942 was studied using a tac promoter and the lacIQ system. Over-expression was induced with 40 microgram/ml IPTG in the growth medium for either 6 or 12 h at growth irradiance (50 mumol photons m-2 s-1). This treatment doubled the amount of psbAII/III mRNA and the D1:2 protein in membranes but decreased the amount of psbAI messages and the D1:1 protein. The total amount of both heterodimeric reaction centre proteins, D1 and D2, remained constant under growth light conditions, indicating that the number of PSII centres in the membranes was not affected, only the form of the D1 protein was changed from D1:1 to D1:2 in most centres. When the cells were photoinhibited either at 500 or 1000 mumol photons m-2 s-1, in the presence or absence of the protein synthesis inhibitor lincomycin, the D1:2 protein remained at a higher level in cells in which over-expression had been induced by IPTG. These cells were also less prone to photoinhibition of PSII. It is suggested that the tolerance of cells to photoinhibition increases when most PSII reaction centres contain the D1:2 protein at the beginning of high irradiance. This tolerance is further strengthened by maintaining psbAIII gene over-expression during the photoinhibitory treatment.
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