ArticleNucleic acids research1996
Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- PRPF31-retinitis pigmentosa: Challenges and opportunities for clinical translation.Vision research · 2023Review
- Pre-mRNA Processing Factors and Retinitis Pigmentosa: RNA Splicing and Beyond.Frontiers in cell and developmental biology · 2021Review
- Identification of photoreceptor genes affected by PRPF31 mutations associated with autosomal dominant retinitis pigmentosa.Neurobiology of disease · 2007Article
- Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Evolution in Saccharomyces cerevisiae: identification of mutations increasing fitness in laboratory populations.Genetics · 2003Article
- Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Article
- Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing.Molecular and cellular biology · 1999Article
- Temperature-sensitive splicing in the floral homeotic mutant apetala3-1.The Plant cell · 1998Article
- Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae.Molecular and cellular biology · 1997Article
- The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have identified six new genes whose products are necessary for the splicing of nuclear pre-mRNA in the yeast Saccharomyces cerevisiae. A collection of 426 temperature-sensitive yeast strains was generated by EMS mutagenesis. These mutants were screened for pre-mRNA splicing defects by an RNA gel blot assay, using the intron- containing CRY1 and ACT1 genes as hybridization probes. We identified 20 temperature-sensitive mutants defective in pre-mRNA splicing. Twelve appear to be allelic to the previously identified prp2, prp3, prp6, prp16/prp23, prp18, prp19 or prp26 mutations that cause defects in spliceosome assembly or the first or second step of splicing. One is allelic to SNR14 encoding U4 snRNA. Six new complementation groups, prp29-prp34, were identified. Each of these mutants accumulates unspliced pre-mRNA at 37 degrees C and thus is blocked in spliceosome assembly or early steps of pre-mRNA splicing before the first cleavage and ligation reaction. The prp29 mutation is suppressed by multicopy PRP2 and displays incomplete patterns of complementation with prp2 alleles, suggesting that the PRP29 gene product may interact with that of PRP2. There are now at least 42 different gene products, including the five spliceosomal snRNAs and 37 different proteins that are necessary for pre-mRNA splicing in Saccharomyces cerevisiae. However, the number of yeast genes identifiable by this approach has not yet been exhausted.
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