ArticleNucleic acids research1996
Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Study of Stress Granule Core Protein AtUBP1b PhosphorylationPlants (Basel, Switzerland) · 2025Article
- A genetic screen in Arabidopsis reveals the identical roles for RBP45d and PRP39a in 5' cryptic splice site selection.Frontiers in plant science · 2022Article
- Improved Homologous Expression of the Acidic Lipase fromJournal of microbiology and biotechnology · 2020Article
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- Deciphering the plant splicing code: experimental and computational approaches for predicting alternative splicing and splicing regulatory elements.Frontiers in plant science · 2012Article
- Testing the IMEter on rice introns and other aspects of intron-mediated enhancement of gene expression.Journal of experimental botany · 2011Article
- The pivotal roles of TIA proteins in 5' splice-site selection of alu exons and across evolution.PLoS genetics · 2009Article
- Where do introns come from?PLoS biology · 2008Article
- Plant spliceosomal introns: not only cut and paste.Current genomics · 2008Article
- Determinants of plant U12-dependent intron splicing efficiency.The Plant cell · 2004Article
- Transcription-coupled and splicing-coupled strand asymmetries in eukaryotic genomes.Nucleic acids research · 2004Article
- Article
- Requirements for intron-mediated enhancement of gene expression in Arabidopsis.RNA (New York, N.Y.) · 2002Article
- UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants.Molecular and cellular biology · 2002Article
- Mutational analysis of a plant branchpoint and polypyrimidine tract required for constitutive splicing of a mini-exon.RNA (New York, N.Y.) · 2002Article
- RBP45 and RBP47, two oligouridylate-specific hnRNP-like proteins interacting with poly(A)+ RNA in nuclei of plant cells.RNA (New York, N.Y.) · 2000Article
- UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation.The EMBO journal · 2000Article
- Requirements for mini-exon inclusion in potato invertase mRNAs provides evidence for exon-scanning interactions in plants.RNA (New York, N.Y.) · 2000Article
- Splicing enhancement in the yeast rp51b intron.RNA (New York, N.Y.) · 2000Article
- Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.Plant physiology · 2000Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introns of nuclear pre-mRNAs in dicotyledonous plants, unlike introns in vertebrates or yeast, are distinctly rich in A+U nucleotides and this feature is essential for their processing. In order to define more precisely sequence elements important for intron recognition in plants, we investigated the effects of short insertions, either U-rich or A-rich, on splicing of synthetic introns in transfected protoplast of Nicotiana plumbaginifolia. It was found that insertions of U-rich (sequence UUUUUAU) but not A-rich (AUAAAAA) segments can activate splicing of a GC-rich synthetic infron, and that U-rich segments, or multimers thereof, can function irrespective of the site of insertion within the intron. Insertions of multiple U-rich segments, either at the same or different locations, generally had an additive, stimulatory effect on splicing. Mutational analysis showed that replacement of one or two U residues in the UUUUUAU sequence with A or C residues had only a small effect on splicing, but replacement with G residues was strongly inhibitory. Proteins that interact with fragments of natural and synthetic pre-mRNAs in vitro were identified in nuclear extracts of N.plumbaginifolia by UV cross- linking. The profile of cross-linked plant proteins was considerably less complex than that obtained with a HeLa cell nuclear extract. Two major cross-linkable plant proteins had apparent molecular mass of 50 and 54 kDa and showed affinity for oligouridilates present in synGC introns or for poly(U).
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