ArticleNucleic acids research1996
Base and sugar requirements for RNA cleavage of essential nucleoside residues in internal loop B of the hairpin ribozyme: implications for secondary structure.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Critical structural perturbations of ribozyme active sites induced by 2'-O-methylation commonly used in structural studies.Nucleic acids research · 2026Article
- Identification of over 200-fold more hairpin ribozymes than previously known in diverse circular RNAs.Nucleic acids research · 2021Article
- Crystal structure of Pistol, a class of self-cleaving ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Hidden ribozymes in eukaryotic genome sequence.F1000 biology reports · 2010Article
- Metal ion binding and the folding of the hairpin ribozyme.RNA (New York, N.Y.) · 2002Article
- Comparative analysis of hairpin ribozyme structures and interference data.Nucleic acids research · 2002Article
- Functional involvement of G8 in the hairpin ribozyme cleavage mechanism.The EMBO journal · 2001Article
- Investigation of adenosine base ionization in the hairpin ribozyme by nucleotide analog interference mapping.RNA (New York, N.Y.) · 2001Article
- Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis.Nature · 2001Article
- The folding of the hairpin ribozyme: dependence on the loops and the junction.RNA (New York, N.Y.) · 2000Article
- RNA double cleavage by a hairpin-derived twin ribozyme.Nucleic acids research · 2000Article
- The hairpin ribozyme. Discovery, mechanism, and development for gene therapy.Molecular biotechnology · 1999Review
- The influence of junction conformation on RNA cleavage by the hairpin ribozyme in its natural junction form.RNA (New York, N.Y.) · 1999Article
- Structure-function relationships in the hammerhead ribozyme probed by base rescue.RNA (New York, N.Y.) · 1998Article
- Specific RNA self-cleavage in coconut cadang cadang viroid: potential for a role in rolling circle replication.RNA (New York, N.Y.) · 1998Article
- The loop B domain is physically separable from the loop A domain in the hairpin ribozyme.Nucleic acids research · 1996Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The hairpin ribozyme is a small self-cleaving RNA that can be engineered for RNA cleavage in trans and has potential as a therapeutic agent. We have used a chemical synthesis approach to study the requirements of hairpin RNA cleavage for sugar and base moieties in residues of internal loop B, an essential region in one of the two ribozyme domains. Individual nucleosides were substituted by either a 2'-deoxy-nucleoside, an abasic residue, or a C3-spacer (propyl linker) and the abilities of the modified ribozymes to cleave an RNA substrate were studied in comparison with the wild-type ribozyme. From these results, together with previous studies, we propose a new model for the potential secondary structure of internal loop B of the hairpin ribozyme.
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