ArticleThe Journal of cell biology1996
Contractile activity regulates isoform expression and polysialylation of NCAM in cultured myotubes: involvement of Ca2+ and protein kinase C.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- A stem-cell based bioassay to critically assess the pathology of dysfunctional neuromuscular junctions.PloS one · 2014Article
- Neural cell adhesion molecule-mediated Fyn activation promotes GABAergic synapse maturation in postnatal mouse cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- In vivo activation of channelrhodopsin-2 reveals that normal patterns of spontaneous activity are required for motoneuron guidance and maintenance of guidance molecules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Control of alternative pre-mRNA splicing by Ca(++) signals.Biochimica et biophysica acta · 2008Review
- Selective targeting of different neural cell adhesion molecule isoforms during motoneuron myotube synapse formation in culture and the switch from an immature to mature form of synaptic vesicle cycling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Functional properties of motoneurons derived from mouse embryonic stem cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Distinct roles of different neural cell adhesion molecule (NCAM) isoforms in synaptic maturation revealed by analysis of NCAM 180 kDa isoform-deficient mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Repression of slow myosin heavy chain 2 gene expression in fast skeletal muscle fibers by muscarinic acetylcholine receptor and G(alpha)q signaling.The Journal of cell biology · 2003Article
- Morphofunctional plasticity in the adult hypothalamus induces regulation of polysialic acid-neural cell adhesion molecule through changing activity and expression levels of polysialyltransferases.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- The pattern of avian intramuscular nerve branching is determined by the innervating motoneuron and its level of polysialic acid.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Neuromuscular activity blockade induced by muscimol and d-tubocurarine differentially affects the survival of embryonic chick motoneurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- BEN/SC1/DM-GRASP expression during neuromuscular development: a cell adhesion molecule regulated by innervation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Selective fasciculation and divergent pathfinding decisions of embryonic chick motor axons projecting to fast and slow muscle regions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- The role of glycoproteins in neural development function, and disease.Molecular neurobiology · 1998Review
- Functional N-methyl-D-aspartate receptors in O-2A glial precursor cells: a critical role in regulating polysialic acid-neural cell adhesion molecule expression and cell migration.The Journal of cell biology · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Muscle development involves a series of complex cell-cell interactions that are mediated, at least in part, by several different cell adhesion molecules. Previous work from this lab showed that the different isoforms of NCAM and its level of polysialylation are developmentally regulated during chick myogenesis in vivo and that this regulation is important for normal muscle development. Using developing chick secondary myotubes grown in culture, we show here that both the polysialylation of NCAM and the developmental switch in isoform expression are regulated by activity and that Ca2+ entry through voltage-gated channels and the subsequent activation of protein kinase C are required for the developmental changes in NCAM isoform synthesis. Specifically, PSA expression was shown to be developmentally regulated with high expression being temporally correlated with the onset of spontaneous contractile activity. Furthermore, blocking contractile activity caused a decrease in PSA expression, while increasing activity with electrical stimulation resulted in its up-regulation. Immunoblot and metabolic labeling studies indicated that dividing myoblasts synthesize primarily 145-kD NCAM, newly formed, spontaneously contracting myotubes synthesize 130-, 145-, and 155-kD NCAM isoforms, while older, more mature myotubes primarily synthesize the glycosylphosphatidylinositol-anchored 130-kD isoform which, in contrast to the other three isoforms, had a high rate of turnover. This developmental switch in NCAM isoform expression could be inhibited with Ca2+ channel blockers and inhibitors of protein kinase C. Taken together, these results suggest that Ca2+ ions and protein kinase C are involved in a second messenger cascade coupling membrane depolarization with transcriptional factors that regulate NCAM isoform synthesis and polysialylation.
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