ArticleNucleic acids research1996
Extended interactions between the primer tRNAi(Met) and genomic RNA of the yeast Ty1 retrotransposon.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- RNA Binding Properties of the Ty1 LTR-Retrotransposon Gag Protein.International journal of molecular sciences · 2021Article
- Structure-Function Model for Kissing Loop Interactions That Initiate Dimerization of Ty1 RNA.Viruses · 2017Article
- Reverse Transcription in the Saccharomyces cerevisiae Long-Terminal Repeat Retrotransposon Ty3.Viruses · 2017Review
- Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3.Viruses · 2016Review
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- A simple and efficient method to isolate LTR sequences of plant retrotransposon.BioMed research international · 2014Article
- Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription.RNA (New York, N.Y.) · 2013Article
- Influence of RNA structural elements on Ty1 retrotransposition.Mobile genetic elements · 2013Article
- Exploring Ty1 retrotransposon RNA structure within virus-like particles.Nucleic acids research · 2013Article
- Host co-factors of the retrovirus-like transposon Ty1.Mobile DNA · 2012Article
- A sequence similar to tRNA 3 Lys gene is embedded in HIV-1 U3-R and promotes minus-strand transfer.Nature structural & molecular biology · 2010Article
- A new family of Ty1-copia-like retrotransposons originated in the tomato genome by a recent horizontal transfer event.Genetics · 2009Article
- The diversity of retrotransposons and the properties of their reverse transcriptases.Virus research · 2008Review
- Characterization of a nucleocapsid-like region and of two distinct primer tRNALys,2 binding sites in the endogenous retrovirus Gypsy.Nucleic acids research · 2006Article
- Identification and characterization of critical cis-acting sequences within the yeast Ty1 retrotransposon.RNA (New York, N.Y.) · 2005Article
- Synthetic tRNALys,3 as the replication primer for the HIV-1HXB2 and HIV-1Mal genomes.Nucleic acids research · 2004Article
- Structure-function relationships of the initiation complex of HIV-1 reverse transcription: the case of mutant viruses using tRNA(His) as primer.Nucleic acids research · 2003Article
- On the importance of the primer activation signal for initiation of tRNA(lys3)-primed reverse transcription of the HIV-1 RNA genome.Nucleic acids research · 2003Article
- Does the HIV-1 primer activation signal interact with tRNA3(Lys) during the initiation of reverse transcription?Nucleic acids research · 2003Article
- A 5'-3' long-range interaction in Ty1 RNA controls its reverse transcription and retrotransposition.The EMBO journal · 2002Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription of the yeast Ty1 retrotransposon is primed by tRNAi(Met) base paired to the primer binding site near the 5'-end of Ty1 genomic RNA. To understand the molecular basis of the tRNAi(Met)-Ty1 RNA interaction the secondary structure of the binary complex was analysed. Enzymatic probes were used to test the conformation of tRNAi(Met) and of Ty1 RNA in the free form and in the complex. A secondary structure model of the tRNAi(Met) Ty1 RNA complex consistent with the probing data was constructed with the help of a computer program. The model shows that besides interactions between the primer binding site and the last 10 nt at the 3'-end of tRNAi(Met), three short regions of Ty1 RNA named boxes 0, 1 and 2.1 interact with the T and D stems and loops of tRNAiMet. Mutations were made in the boxes or in the complementary sequences of tRNAi(Met) to study the contribution of these sequences to formation of the complex. We find that interaction with at least one of the two boxes 0 or 1 is absolutely required for efficient annealing of the two RNAs. Sequence comparison showing that the primary sequence of the boxes is strictly conserved in Ty1 and Ty2 elements and previously published in vivo results underline the functional importance of the primary sequence of the boxes and suggest that extended interactions between genomic Ty1 RNA and the primary tRNAi(Met) play a role in the reverse transcription pathway.
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