ArticleGenetics1995
Molecular and genetic analysis of the toxic effect of RAP1 overexpression in yeast.
Article in Genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- Stress-driven emergence of heritable non-genetic drug resistance.Research square · 2025Article
- Rap1 overexpression boosts triterpenoid saponin production in yeast by enhancing precursor supply and heterologous gene expression.Microbial cell factories · 2025Article
- Benchmarking recent computational tools for DNA-binding protein identification.Briefings in bioinformatics · 2024Article
- Genetically stable kill-switch using "demon and angel" expression construct of essential genes.Frontiers in bioengineering and biotechnology · 2024Article
- Comparative Analysis on Alignment-Based and Pretrained Feature Representations for the Identification of DNA-Binding Proteins.Computational and mathematical methods in medicine · 2022Article
- DP-BINDER: machine learning model for prediction of DNA-binding proteins by fusing evolutionary and physicochemical information.Journal of computer-aided molecular design · 2019Article
- Functional Site Discovery From Incomplete Training Data: A Case Study With Nucleic Acid-Binding Proteins.Frontiers in genetics · 2019Article
- Repression of Divergent Noncoding Transcription by a Sequence-Specific Transcription Factor.Molecular cell · 2018Article
- iDNAProt-ES: Identification of DNA-binding Proteins Using Evolutionary and Structural Features.Scientific reports · 2017Article
- Identification of DNA-binding proteins using multi-features fusion and binary firefly optimization algorithm.BMC bioinformatics · 2016Article
- The wrapping loop and Rap1 C-terminal (RCT) domain of yeast Rap1 modulate access to different DNA binding modes.The Journal of biological chemistry · 2015Article
- The DNA-binding domain of yeast Rap1 interacts with double-stranded DNA in multiple binding modes.Biochemistry · 2014Article
- Sequence based prediction of DNA-binding proteins based on hybrid feature selection using random forest and Gaussian naïve Bayes.PloS one · 2014Article
- Rap1 relocalization contributes to the chromatin-mediated gene expression profile and pace of cell senescence.Genes & development · 2013Article
- The orientation of the C-terminal domain of the Saccharomyces cerevisiae Rap1 protein is determined by its binding to DNA.Nucleic acids research · 2012Article
- Boosting the prediction and understanding of DNA-binding domains from sequence.Nucleic acids research · 2010Article
- The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein.The Journal of biological chemistry · 2008Article
- Learning to translate sequence and structure to function: identifying DNA binding and membrane binding proteins.Annals of biomedical engineering · 2007Article
- Genetic dissection of the Kluyveromyces lactis telomere and evidence for telomere capping defects in TER1 mutants with long telomeres.Eukaryotic cell · 2004Article
- Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.Genome biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rap1p is a context-dependent regulatory protein in yeast that functions as a transcriptional activator of many essential genes, including those encoding ribosomal proteins and glycolytic enzymes. Rap1p also participates in transcriptional silencing at HM mating-type loci and telomeres. Overexpression of RAP1 strongly inhibits cell growth, perhaps by interfering with essential transcriptional activation functions within the cell. Here we report a molecular and genetic analysis of the toxic effect of RAP1 overexpression. We show that toxicity does not require the previously defined Rap1p activation and silencing domains, but instead is dependent upon the DNA-binding domain and an adjacent region of unknown function. Point mutations were identified in the DNA-binding domain that relieve the toxic effect of overexpression. Two of these mutations can complement a RAP1 deletion yet cause growth defects and altered DNA-binding properties in vitro. However, a small deletion of the adjacent (downstream) region that abolishes overexpression toxicity has, by itself, no apparent effect on growth or DNA binding. SKO1/ACR1, which encodes a CREB-like repressor protein in yeast, was isolated as a high copy suppressor of the toxicity caused by RAP1 overexpression. Models related to the regulation of Rap1p activity are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.