ArticleRNA (New York, N.Y.)1996
Loop IV of 5S ribosomal RNA has contacts both to domain II and to domain V of the 23S RNA.
Article in RNA (New York, N.Y.), 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed.
- Cryo-electron microscopy visualization of a large insertion in the 5S ribosomal RNA of the extremely halophilic archaeon Halococcus morrhuae.FEBS open bio · 2020Article
- Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.Nature communications · 2020Article
- Proteome evolution and the metabolic origins of translation and cellular life.Journal of molecular evolution · 2011Article
- The evolutionary history of the structure of 5S ribosomal RNA.Journal of molecular evolution · 2009Article
- Ribosome performance is enhanced by a rich cluster of pseudouridines in the A-site finger region of the large subunit.The Journal of biological chemistry · 2008Article
- Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli.Journal of molecular biology · 2007Article
- Changes in the conformation of 5S rRNA cause alterations in principal functions of the ribosomal nanomachine.Nucleic acids research · 2007Article
- Structural and functional analysis of 5S rRNA in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2005Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- 5S rRNA: Structure and Function from Head to Toe.International journal of biomedical science : IJBS · 2005Article
- 5 S rRNA: structure and interactions.The Biochemical journal · 2003Review
- Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.RNA (New York, N.Y.) · 2002Article
- Mining biochemical information: lessons taught by the ribosome.RNA (New York, N.Y.) · 2002Article
- Saturation mutagenesis of 5S rRNA in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge.RNA (New York, N.Y.) · 2000Article
- Structure of 5S rRNA within the Escherichia coli ribosome: iodine-induced cleavage patterns of phosphorothioate derivatives.RNA (New York, N.Y.) · 1998Article
- New features of 23S ribosomal RNA folding: the long helix 41-42 makes a "U-turn" inside the ribosome.RNA (New York, N.Y.) · 1998Article
- The environment of 5S rRNA in the ribosome: cross-links to the GTPase-associated area of 23S rRNA.Nucleic acids research · 1998Article
- A new technique for the characterization of long-range tertiary contacts in large RNA molecules: insertion of a photolabel at a selected position in 16S rRNA within the Escherichia coli ribosome.Nucleic acids research · 1997Article
- Molecular genetics of the peptidyl transferase center and the unusual Var1 protein in yeast mitochondrial ribosomes.Experientia · 1996Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An analogue of 5S rRNA, containing a random distribution of thiouridine residues in place of normal uridine, was prepared by T7 transcription from a suitable DNA template. The modified RNA molecule was reconstituted into 50S or 70S ribosomes, and the thiouridine residues were activated by irradiation at 350 nm. Crosslinks generated between the 5S and 23S RNA were analyzed by our standard procedures. Two crosslink sites were identified, one to residue A-960 at the loop-end of helix 39 in Domain II, and the other to C-2475 at the loop-end of helix 89 in Domain V of the 23S RNA. Both crosslinks involved residue U-89 of the 5S RNA, that in Domain V corresponding to the principal crosslink found in a previously published series of experiments. The relative intensities of the two crosslink sites were found to be highly dependent on individual preparations of 50S ribosomal proteins and 23S RNA. The results are discussed in terms of the three-dimensional folding and dynamics of the 23S RNA within the 50S subunit.
Indexed as
Identifiers
8601281PMC1369359What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.